8TIP

Isoreticular, interpenetrating co-crystal of Replication Initiator Protein REPE54 and symmetrical expanded duplex (31mer) containing the cognate REPE54 sequence and an additional G-C rich sequence with blunt ends and 5' terminal phosphates.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	DNA (5'-D(A*CP*CP*CP*GP*GP*AP*CP*CP*TP*GP*TP*GP*AP*CP*AP*AP*AP*TP*TP*GP*CP*CP*CP*TP*CP*AP*GP*AP*CP*GP*G)-3')|	polymer	32	9796.3	1		Escherichia coli
2	B (B)	DNA (5'-D(A*CP*CP*GP*TP*CP*TP*GP*AP*GP*GP*GP*CP*AP*AP*TP*TP*TP*GP*TP*CP*AP*CP*AP*GP*GP*TP*CP*CP*GP*GP*G)-3')	polymer	32	9898.4	1		Escherichia coli
3	C (C)	Replication initiation protein	polymer	263	30749.1	1	UniProt (P03856) Pfam (PF01051) Pfam (PF21205)	Escherichia coli K-12	Protein E,Protein rep,Protein F4
4	D, E, F, G, H (A, B, C)	MAGNESIUM ION	non-polymer		24.3	5	Chemie (MG)
5	I, J, K (A, B, C)	water	water		18.0	16	Chemie (HOH)

Sequence modifications

C: 1 - 251 (UniProt: P03856)

PDB	External Database	Details
Met -11	-	initiating methionine
Arg -10	-	expression tag
Gly -9	-	expression tag
Ser -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag
Pro 118	Arg 118	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	50443.7
Non-Polymers*	Number of molecules	5
	Total formula weight	121.5
All*	Total formula weight	50565.2

*Water molecules are not included.