8THI

Cryo-EM structure of the Tripartite ATP-independent Periplasmic (TRAP) transporter SiaQM from Haemophilus influenzae (parallel dimer)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Sialic acid TRAP transporter permease protein SiaT	polymer	655	72046.8	2	UniProt (P44543) Pfam (PF04290) Pfam (PF06808)	Haemophilus influenzae Rd KW20	N-acetylneuraminic acid permease,N-acetylneuraminic acid transporter,Neu5Ac permease
2	C, E (A, B)	(1S)-2-{[{[(2R)-2,3-DIHYDROXYPROPYL]OXY}(HYDROXY)PHOSPHORYL]OXY}-1-[(PALMITOYLOXY)METHYL]ETHYL STEARATE	non-polymer		751.0	2	Chemie (PGT)
3	D, F (A, B)	SODIUM ION	non-polymer		23.0	2	Chemie (NA)

Sequence modifications

A, B: 1 - 616 (UniProt: P44543)

PDB	External Database	Details
Met -38	-	initiating methionine
Gly -37	-	expression tag
Gly -36	-	expression tag
Ser -35	-	expression tag
His -34	-	expression tag
His -33	-	expression tag
His -32	-	expression tag
His -31	-	expression tag
His -30	-	expression tag
His -29	-	expression tag
Gly -28	-	expression tag
Met -27	-	expression tag
Ala -26	-	expression tag
Ser -25	-	expression tag
Met -24	-	expression tag
Thr -23	-	expression tag
Gly -22	-	expression tag
Gly -21	-	expression tag
Gln -20	-	expression tag
Gln -19	-	expression tag
Met -18	-	expression tag
Gly -17	-	expression tag
Arg -16	-	expression tag
Asp -15	-	expression tag
Leu -14	-	expression tag
Tyr -13	-	expression tag
Asp -12	-	expression tag
Asp -11	-	expression tag
Asp -10	-	expression tag
Asp -9	-	expression tag
Lys -8	-	expression tag
Asp -7	-	expression tag
Arg -6	-	expression tag
Trp -5	-	expression tag
Gly -4	-	expression tag
Ser -3	-	expression tag
Glu -2	-	expression tag
Leu -1	-	expression tag
Glu 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	144093.6
Non-Polymers*	Number of molecules	4
	Total formula weight	1548.0
All*	Total formula weight	145641.7

*Water molecules are not included.