8THE
Cryo-EM structure of Pseudomonas aeruginosa TonB-dependent transporter PhuR in complex with synthetic antibody and heme
This is a non-PDB format compatible entry.
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Heme/hemoglobin uptake outer membrane receptor PhuR | polymer | 777 | 86370.3 | 1 | UniProt (Q9HV88) Pfam (PF07715) Pfam (PF00593) | Pseudomonas aeruginosa | |
| 2 | B (H) | Synthetic Antibody Heavy Chain | polymer | 238 | 25525.4 | 1 | Homo sapiens (human) | ||
| 3 | C (L) | Synthetic Antibody Light Chain | polymer | 215 | 23212.7 | 1 | Homo sapiens (human) | ||
| 4 | D (A) | PROTOPORPHYRIN IX CONTAINING FE | non-polymer | 616.5 | 1 | Chemie (HEM) | |||
| 5 | E (A) | (2~{R},4~{R},5~{S},6~{S})-6-[(1~{R})-1,2-bis(oxidanyl)ethyl]-2-[[(2~{R},3~{S},4~{S},5~{R},6~{S})-5-[[(3~{R})-3-dodecanoyloxytetradecanoyl]amino]-6-[[(2~{R},3~{R},4~{R},5~{S},6~{S})-3-oxidanyl-5-[[(3~{R})-3-oxidanyltetradecanoyl]amino]-4-[(3~{R})-3-oxidanyltetradecanoyl]oxy-6-phosphonooxy-oxan-2-yl]methoxy]-3-phosphonooxy-4-[(3~{S})-3-tetradecanoyloxytetradecanoyl]oxy-oxan-2-yl]methoxy]-4,5-bis(oxidanyl)oxane-2-carboxylic acid | non-polymer | 2018.5 | 1 | Chemie (CTQ) | |||
| 6 | F, G, H (A, H, L) | water | water | 18.0 | 81 | Chemie (HOH) |
Sequence modifications
A: 26 - 764 (UniProt: Q9HV88)
| PDB | External Database | Details |
|---|---|---|
| Met -12 | - | initiating methionine |
| Lys -11 | - | expression tag |
| Tyr -10 | - | expression tag |
| Leu -9 | - | expression tag |
| Leu -8 | - | expression tag |
| Pro -7 | - | expression tag |
| Thr -6 | - | expression tag |
| Ala -5 | - | expression tag |
| Ala -4 | - | expression tag |
| Ala -3 | - | expression tag |
| Gly -2 | - | expression tag |
| Leu -1 | - | expression tag |
| Leu 0 | - | expression tag |
| Leu 1 | - | expression tag |
| Leu 2 | - | expression tag |
| Ala 3 | - | expression tag |
| Ala 4 | - | expression tag |
| Gln 5 | - | expression tag |
| Pro 6 | - | expression tag |
| Ala 7 | - | expression tag |
| Met 8 | - | expression tag |
| Ala 9 | - | expression tag |
| Met 10 | - | expression tag |
| Gly 11 | - | expression tag |
| His 12 | - | expression tag |
| His 13 | - | expression tag |
| His 14 | - | expression tag |
| His 15 | - | expression tag |
| His 16 | - | expression tag |
| His 17 | - | expression tag |
| Gly 18 | - | expression tag |
| Glu 19 | - | expression tag |
| Asn 20 | - | expression tag |
| Leu 21 | - | expression tag |
| Tyr 22 | - | expression tag |
| Phe 23 | - | expression tag |
| Gln 24 | - | expression tag |
| Gly 25 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 3 |
| Total formula weight | 135108.5 | |
| Non-Polymers* | Number of molecules | 2 |
| Total formula weight | 2635.0 | |
| All* | Total formula weight | 137743.5 |
