8TH9

Structure of mammalian NEIL2 from Monodelphis domestica in complex with THF-containing DNA

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	DNA-(apurinic or apyrimidinic site) lyase	polymer	290	32864.2	1	UniProt (F7AMK3) Pfam (PF06831) In PDB	Monodelphis domestica (gray short-tailed opossum)	NEIL2 DNA Glycosylase
2	C	DNA (5'-D(*GP*TP*AP*GP*AP*CP*CP*TP*GP*GP*AP*CP*G)-3')	polymer	13	4016.6	1		synthetic construct
3	D	DNA (5'-D(*GP*CP*GP*TP*CP*CP*AP*(3DR)P*GP*TP*CP*TP*AP*C)-3')	polymer	14	4107.7	1		synthetic construct
4	A	ZINC ION	non-polymer		65.4	1	Chemie (ZN)
5	A	DIMETHYL SULFOXIDE	non-polymer		78.1	1	Chemie (DMS)
6		water	water		18.0	59	Chemie (HOH)

Sequence modifications

A: 2 - 120 (UniProt: F7AMK3)
A: 126 - 336 (UniProt: F7AMK3)

PDB	External Database	Details
Gly 121	-	linker
Ser 122	-	linker
Gly 123	-	linker
Ser 124	-	linker
Gly 125	-	linker
Leu 337	-	expression tag
Glu 338	-	expression tag
His 339	-	expression tag
His 340	-	expression tag
His 341	-	expression tag
His 342	-	expression tag
His 343	-	expression tag
His 344	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	40988.5
Non-Polymers*	Number of molecules	2
	Total formula weight	143.5
All*	Total formula weight	41132.0

*Water molecules are not included.