8TGV

CryoEM structure of Fab HC84.26-HCV E2 complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, D, G	envelope glycoprotein E2	polymer	291	32287.3	3	UniProt (X4ZFZ7) Pfam (PF01560) In PDB	Hepacivirus C
2	E, B, I, K	HC84.26 Heavy chain	polymer	255	27304.6	4		Homo sapiens (human)
3	F, C, J, L	HC84.26 Light chain	polymer	234	25352.2	4		Homo sapiens (human)
4	Z	beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		586.5	1	In PDB GlyTouCan (G15407YE)
5	H, M, N	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	3	In PDB GlyTouCan (G42666HT)
6	A	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	1	Chemie (NAG)

Sequence modifications

A, D, G: 384 - 645 (UniProt: X4ZFZ7)

PDB	External Database	Details
Met 363	-	initiating methionine
Glu 364	-	expression tag
Thr 365	-	expression tag
Asp 366	-	expression tag
Thr 367	-	expression tag
Leu 368	-	expression tag
Leu 369	-	expression tag
Leu 370	-	expression tag
Trp 371	-	expression tag
Val 372	-	expression tag
Leu 373	-	expression tag
Leu 374	-	expression tag
Leu 375	-	expression tag
Trp 376	-	expression tag
Val 377	-	expression tag
Pro 378	-	expression tag
Gly 379	-	expression tag
Ser 380	-	expression tag
Thr 381	-	expression tag
Gly 382	-	expression tag
Asp 383	-	expression tag
Ile 646	-	expression tag
Gly 647	-	expression tag
His 648	-	expression tag
His 649	-	expression tag
His 650	-	expression tag
His 651	-	expression tag
His 652	-	expression tag
His 653	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	11
	Total formula weight	307488.8
Branched	Number of molecules	4
	Total formula weight	1859.7
Non-Polymers*	Number of molecules	1
	Total formula weight	221.2
All*	Total formula weight	309569.8

*Water molecules are not included.