8TG4

tRNA 2'-phosphotransferase (Tpt1) from Aeropyrum pernix in complex with ADP-ribose-2"-phosphate and 2'-OH RNA

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Probable RNA 2'-phosphotransferase	polymer	206	23382.0	1	UniProt (Q9YFP5) Pfam (PF01885)	Aeropyrum pernix
2	B (C)	RNA (5'-R(*UP*GP*UP*A)-3')	polymer	5	1548.0	1		synthetic construct
3	C (A)	NITRATE ION	non-polymer		62.0	1	Chemie (NO3)
4	D (A)	1,2-ETHANEDIOL	non-polymer		62.1	1	Chemie (EDO)
5	E (A)	[(2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-3,4-dihydroxyoxolan-2-yl]methyl [(2R,3R,4R,5R)-3,5-dihydroxy-4-(phosphonooxy)oxolan-2-yl]methyl dihydrogen diphosphate (non-preferred name)	non-polymer		639.3	1	Chemie (9SO)
6	F, G, H, I (A)	POTASSIUM ION	non-polymer		39.1	4	Chemie (K)
7	J, K (A)	CHLORIDE ION	non-polymer		35.5	2	Chemie (CL)
8	L, M (A, C)	water	water		18.0	145	Chemie (HOH)

Sequence modifications

A: 1 - 186 (UniProt: Q9YFP5)

PDB	External Database	Details
Met -19	-	expression tag
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	24930.0
Non-Polymers*	Number of molecules	9
	Total formula weight	990.7
All*	Total formula weight	25920.6

*Water molecules are not included.