8TB9

PRC2-J119-450 monomer bound to H1-nucleosome

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Histone H1.0	polymer	197	21209.5	1	UniProt (P07305) Pfam (PF00538)	Homo sapiens (human)	Histone H1',Histone H1(0)
2	B (B)	Protein Jumonji	polymer	334	36251.7	1	UniProt (Q92833)	Homo sapiens (human)	Jumonji/ARID domain-containing protein 2
3	C (D)	Polycomb protein SUZ12	polymer	619	72266.4	1	UniProt (Q15022) Pfam (PF09733)	Homo sapiens (human)	Chromatin precipitated E2F target 9 protein,ChET 9 protein,Joined to JAZF1 protein,Suppressor of zeste 12 protein homolog
4	D (E)	Histone-lysine N-methyltransferase EZH2	polymer	753	86175.0	1	UniProt (Q15910) Pfam (PF11616) Pfam (PF18118) Pfam (PF21358) Pfam (PF18264) Pfam (PF00856)	Homo sapiens (human)	ENX-1,Enhancer of zeste homolog 2,Lysine N-methyltransferase 6
5	E (G)	Polycomb protein EED	polymer	441	50267.7	1	UniProt (O75530) Pfam (PF00400)	Homo sapiens (human)	hEED,Embryonic ectoderm development protein,WD protein associating with integrin cytoplasmic tails 1,WAIT-1
6	F (H)	DNA (226-MER)	polymer	215	66539.3	1		synthetic construct
7	G, O (I, W)	Histone H3.2	polymer	136	15435.1	2	UniProt (P84233) Pfam (PF00125)	Xenopus laevis (African clawed frog)
8	H, P (J, X)	Histone H4	polymer	106	11625.6	2	UniProt (P62799) Pfam (PF15511)	Xenopus laevis (African clawed frog)
9	I (O)	Histone-binding protein RBBP4	polymer	425	47709.5	1	UniProt (Q09028) Pfam (PF12265) Pfam (PF00400)	Homo sapiens (human)	Chromatin assembly factor 1 subunit C,CAF-1 subunit C,Chromatin assembly factor I p48 subunit,CAF-I 48 kDa subunit,CAF-I p48,Nucleosome-remodeling factor subunit RBAP48,Retinoblastoma-binding protein 4,RBBP-4,Retinoblastoma-binding protein p48
10	J, M (R, U)	Histone H2A type 1	polymer	133	14355.7	2	UniProt (P06897) Pfam (PF00125) Pfam (PF16211)	Xenopus laevis (African clawed frog)
11	K, N (S, V)	Histone H2B 1.1	polymer	123	13655.9	2	UniProt (P02281) Pfam (PF00125)	Xenopus laevis (African clawed frog)	H2B1.1
12	L (T)	DNA (226-MER)	polymer	215	65905.9	1		synthetic construct
13	Q (Y)	Zinc finger protein AEBP2	polymer	303	34183.2	1	UniProt (Q6ZN18)	Homo sapiens (human)	Adipocyte enhancer-binding protein 2,AE-binding protein 2
14	R (E)	S-ADENOSYL-L-HOMOCYSTEINE	non-polymer		384.4	1	Chemie (SAH)

Sequence modifications

A: 0 - 193 (UniProt: P07305)

PDB	External Database	Details
Gly -3	-	expression tag
Ser -2	-	expression tag
His -1	-	expression tag

B: 119 - 450 (UniProt: Q92833)

PDB	External Database	Details
Met 117	-	initiating methionine
Val 118	-	expression tag

D: 68 - 685 (UniProt: Q15022)

PDB	External Database	Details
Met 67	-	initiating methionine

E: 2 - 751 (UniProt: Q15910)

PDB	External Database	Details
Ser -1	-	expression tag
Asn 0	-	expression tag
Ala 1	-	expression tag
Ala 14	Cys 14	conflict
Cys 15	Trp 15	conflict

I, W: 0 - 135 (UniProt: P84233)

PDB	External Database	Details
Ala 102	Gly 103	conflict

J, X: 0 - 102 (UniProt: P62799)

PDB	External Database	Details
Ser 103	-	expression tag
Ser 104	-	expression tag
Gly 105	-	expression tag

R, U: 0 - 129 (UniProt: P06897)

PDB	External Database	Details
Ser -3	-	expression tag
Asn -2	-	expression tag
Ala -1	-	expression tag
Arg 99	Gly 100	conflict
Cys 119	Lys 120	conflict
Ser 123	Ala 124	conflict

S, V: 1 - 122 (UniProt: P02281)

PDB	External Database	Details
Met 0	-	initiating methionine
Thr 29	Ser 33	conflict

Y: 10 - 309 (UniProt: Q6ZN18)

PDB	External Database	Details
Ser 7	-	expression tag
Asn 8	-	expression tag
Ala 9	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	17
	Total formula weight	590652.8
Non-Polymers*	Number of molecules	1
	Total formula weight	384.4
All*	Total formula weight	591037.2

*Water molecules are not included.