8T9H

Catalytic and non-catalytic mechanisms of histone H4 lysine 20 methyltransferase SUV420H1

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, E (A, E)	Histone H3.2	polymer	135	15303.9	2	UniProt (P84233) Pfam (PF00125)	Xenopus laevis (African clawed frog)	Histone H3
2	B, F (B, F)	Histone H4	polymer	103	11396.4	2	UniProt (A0A8J1LTD2) Pfam (PF15511) UniProt (by SIFTS) (P62799)	Xenopus laevis (African clawed frog)
3	C, G (C, G)	Histone H2A	polymer	130	14109.4	2	UniProt (Q6AZJ8) Pfam (PF00125) Pfam (PF16211) UniProt (by SIFTS) (P06897)	Xenopus laevis (African clawed frog)
4	D, H (D, H)	Histone H2B 1.1	polymer	123	13655.9	2	UniProt (P02281) Pfam (PF00125)	Xenopus laevis (African clawed frog)	H2B1.1
5	I (I)	DNA (146-MER)	polymer	146	44824.6	1		Escherichia coli 'BL21-Gold(DE3)pLysS AG'
6	J (J)	DNA (146-MER)	polymer	146	45304.9	1		Escherichia coli 'BL21-Gold(DE3)pLysS AG'
7	K (K)	Histone-lysine N-methyltransferase KMT5B	polymer	394	44703.1	1	UniProt (Q4FZB7) Pfam (PF00856)	Homo sapiens (human)

Sequence modifications

A, E: 1 - 135 (UniProt: P84233)

PDB	External Database	Details
Ala 102	Gly 103	variant

B, F: 0 - 102 (UniProt: A0A8J1LTD2)

PDB	External Database	Details
Met 20	Lys 34	conflict

D, H: 4 - 125 (UniProt: P02281)

PDB	External Database	Details
Met 3	-	initiating methionine
Thr 32	Ser 33	engineered mutation

K: 2 - 393 (UniProt: Q4FZB7)

PDB	External Database	Details
Gly 0	-	expression tag
Ser 1	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	11
	Total formula weight	243764.0
All*	Total formula weight	243764.0

*Water molecules are not included.