8T9F

Catalytic and non-catalytic mechanisms of histone H4 lysine 20 methyltransferase SUV420H1

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (K)	Histone-lysine N-methyltransferase KMT5B	polymer	393	44690.2	1	UniProt (Q4FZB7) Pfam (PF00856)	Homo sapiens (human)	Lysine N-methyltransferase 5B,Lysine-specific methyltransferase 5B,Suppressor of variegation 4-20 homolog 1,Su(var)4-20 homolog 1,Suv4-20h1,[histone H4]-N-methyl-L-lysine20 N-methyltransferase KMT5B,[histone H4]-lysine20 N-methyltransferase KMT5B
2	B (I)	DNA (122-MER)	polymer	146	44824.6	1		Escherichia coli 'BL21-Gold(DE3)pLysS AG'
3	C (J)	DNA (122-MER)	polymer	146	45304.9	1		Escherichia coli 'BL21-Gold(DE3)pLysS AG'
4	D, F (B, F)	Histone H4	polymer	103	11396.4	2	UniProt (A0A8J1LTD2) Pfam (PF15511) UniProt (by SIFTS) (P62799)	Xenopus laevis (African clawed frog)
5	E, I (A, E)	Histone H3.2	polymer	135	15303.9	2	UniProt (P84233) Pfam (PF00125)	Xenopus laevis (African clawed frog)	Histone H3
6	G, J (G, C)	Histone H2A.Z	polymer	128	13581.8	2	UniProt (P0C0S5) Pfam (PF00125) Pfam (PF16211)	Homo sapiens (human)	H2A/z
7	H, K (H, D)	Histone H2B 1.1	polymer	123	13655.9	2	UniProt (P02281) Pfam (PF00125)	Xenopus laevis (African clawed frog)	H2B1.1
8	L (K)	S-ADENOSYLMETHIONINE	non-polymer		398.4	1	Chemie (SAM)

Sequence modifications

B, F: 0 - 102 (UniProt: A0A8J1LTD2)

PDB	External Database	Details
Met 20	Lys 34	conflict

A, E: 1 - 135 (UniProt: P84233)

PDB	External Database	Details
Ala 102	Gly 103	variant

H, D: 5 - 126 (UniProt: P02281)

PDB	External Database	Details
Met 4	-	initiating methionine
Thr 33	Ser 33	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	11
	Total formula weight	242695.8
Non-Polymers*	Number of molecules	1
	Total formula weight	398.4
All*	Total formula weight	243094.3

*Water molecules are not included.