8T7A

Cryo-EM structure of RSV preF in complex with Fab 2.4K

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C	Fusion glycoprotein F2	polymer	84	9498.8	3	UniProt (P12568) In PDB	Respiratory syncytial virus	F2
2	a, b, c	Fusion glycoprotein F1	polymer	436	48295.9	3	UniProt (P12568) Pfam (PF00523) In PDB	Respiratory syncytial virus	F1
3	D, F, H	2.4K Fab Heavy Chain	polymer	125	13694.2	3		Homo sapiens
4	E, G, I	2.4K Fab Light Chain	polymer	113	12447.9	3		Homo sapiens
5	a, b, c	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	3	Chemie (NAG)

Sequence modifications

A, B, C: 26 - 109 (UniProt: P12568)

PDB	External Database	Details
Ile 67	Asn 67	conflict
Lys 80	Asn 80	conflict
Pro 101	Thr 101	conflict

a, b, c: 137 - 513 (UniProt: P12568)

PDB	External Database	Details
Val 152	Ile 152	conflict
Ser 213	Arg 213	conflict
Pro 215	Ser 215	conflict
Gly 514	-	expression tag
Ser 515	-	expression tag
Gly 516	-	expression tag
Tyr 517	-	expression tag
Ile 518	-	expression tag
Pro 519	-	expression tag
Glu 520	-	expression tag
Ala 521	-	expression tag
Pro 522	-	expression tag
Arg 523	-	expression tag
Asp 524	-	expression tag
Gly 525	-	expression tag
Gln 526	-	expression tag
Ala 527	-	expression tag
Tyr 528	-	expression tag
Val 529	-	expression tag
Arg 530	-	expression tag
Lys 531	-	expression tag
Asp 532	-	expression tag
Gly 533	-	expression tag
Glu 534	-	expression tag
Trp 535	-	expression tag
Val 536	-	expression tag
Leu 537	-	expression tag
Leu 538	-	expression tag
Ser 539	-	expression tag
Thr 540	-	expression tag
Phe 541	-	expression tag
Leu 542	-	expression tag
Gly 543	-	expression tag
Arg 544	-	expression tag
Ser 545	-	expression tag
Leu 546	-	expression tag
Glu 547	-	expression tag
Val 548	-	expression tag
Leu 549	-	expression tag
Phe 550	-	expression tag
Gln 551	-	expression tag
Gly 552	-	expression tag
Pro 553	-	expression tag
Gly 554	-	expression tag
His 555	-	expression tag
His 556	-	expression tag
His 557	-	expression tag
His 558	-	expression tag
His 559	-	expression tag
His 560	-	expression tag
His 561	-	expression tag
His 562	-	expression tag
Ser 563	-	expression tag
Ala 564	-	expression tag
Trp 565	-	expression tag
Ser 566	-	expression tag
His 567	-	expression tag
Pro 568	-	expression tag
Gln 569	-	expression tag
Phe 570	-	expression tag
Glu 571	-	expression tag
Lys 572	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	12
	Total formula weight	251810.2
Non-Polymers*	Number of molecules	3
	Total formula weight	663.6
All*	Total formula weight	252473.8

*Water molecules are not included.