8T6X

SpRY-Cas9:gRNA complex targeting TAC PAM DNA with 18 bp R-loop

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	CRISPR-associated endonuclease Cas9/Csn1	polymer	1369	159149.4	1	UniProt (Q99ZW2) Pfam (PF16593) Pfam (PF16592) Pfam (PF13395) Pfam (PF22702) Pfam (PF16595)	Streptococcus pyogenes	SpCas9,SpyCas9
2	B (B)	gRNA	polymer	96	31028.5	1		Streptococcus pyogenes
3	C (C)	TS	polymer	55	16920.8	1		Escherichia phage Lambda
4	D (D)	NTS	polymer	55	16970.9	1		Escherichia phage Lambda
5	E, F, G (A, C)	MAGNESIUM ION	non-polymer		24.3	3	Chemie (MG)

Sequence modifications

A: 2 - 1368 (UniProt: Q99ZW2)

PDB	External Database	Details
Glu 1	-	expression tag
Arg 61	Ala 61	engineered mutation
Arg 1111	Leu 1111	engineered mutation
Leu 1135	Asp 1135	engineered mutation
Trp 1136	Ser 1136	engineered mutation
Lys 1218	Gly 1218	engineered mutation
Gln 1219	Glu 1219	engineered mutation
Arg 1317	Asn 1317	engineered mutation
Arg 1322	Ala 1322	engineered mutation
Pro 1333	Arg 1333	engineered mutation
Gln 1335	Arg 1335	engineered mutation
Arg 1337	Thr 1337	engineered mutation
Gly 1369	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	224069.7
Non-Polymers*	Number of molecules	3
	Total formula weight	72.9
All*	Total formula weight	224142.6

*Water molecules are not included.