8T6K

Cryo-EM structure of tetradecameric CaMKII beta holoenzyme T287A T306A T307A

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E... (A, B, C, D, E...)	Venus-tagged CaMKII Beta Holoenzyme mutant	polymer	815	90846.9	14	UniProt (P42212) UniProt (P08413) Pfam (PF01353) Pfam (PF00069) Pfam (PF08332)	Aequorea victoria (Water jellyfish Mesonema victoria)

Sequence modifications

A, B, C, D, E, F, G, H, I, J, K, L, M, N: -251 - -15 (UniProt: P42212)

PDB	External Database	Details
Met -272	-	initiating methionine
Gly -271	-	expression tag
Ser -270	-	expression tag
Ser -269	-	expression tag
His -268	-	expression tag
His -267	-	expression tag
His -266	-	expression tag
His -265	-	expression tag
His -264	-	expression tag
His -263	-	expression tag
Ser -262	-	expression tag
Ser -261	-	expression tag
Gly -260	-	expression tag
Leu -259	-	expression tag
Val -258	-	expression tag
Pro -257	-	expression tag
Arg -256	-	expression tag
Gly -255	-	expression tag
Ser -254	-	expression tag
His -253	-	expression tag
Val -252	-	expression tag
Leu -207	Phe 46	conflict
Leu -189	Phe 64	conflict
Gly -188	Ser 65	conflict
Leu -185	Val 68	conflict
Ala -181	Ser 72	conflict
Thr -100	Met 153	conflict
Ala -90	Val 163	conflict
Gly -78	Ser 175	conflict
Tyr -50	Thr 203	conflict
Lys -47	Ala 206	conflict
Leu -22	His 231	conflict
Ser -14	-	linker
Gly -13	-	linker
Leu -12	-	linker
Arg -11	-	linker
Ser -10	-	linker
Arg -9	-	linker
Ala -8	-	linker
Gln -7	-	linker
Ala -6	-	linker
Ser -5	-	linker
Asn -4	-	linker
Ser -3	-	linker
Ala -2	-	linker
Val -1	-	linker
Asp 0	-	linker

A, B, C, D, E, F, G, H, I, J, K, L, M, N: 1 - 542 (UniProt: P08413)

PDB	External Database	Details
Ala 287	Thr 287	engineered mutation
Ala 306	Thr 306	engineered mutation
Ala 307	Thr 307	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	14
	Total formula weight	1271856.0
All*	Total formula weight	1271856.0

*Water molecules are not included.