8T48

The N4BP1 CUE-like domain in complex with linear di-Ubiquitin

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Di-Ubiquitin	polymer	172	19307.0	2	UniProt (P0CG48) Pfam (PF00240)	Homo sapiens (human)
2	C, D (C, D)	NEDD4-binding protein 1	polymer	68	7732.7	2	UniProt (O75113)	Homo sapiens (human)	N4BP1
3	E (A)	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
4	F (B)	LITHIUM ION	non-polymer		6.9	1	Chemie (LI)
5	G, H, I, J (A, B, C, D)	water	water		18.0	430	Chemie (HOH)

Sequence modifications

A, B: 1 - 152 (UniProt: P0CG48)

PDB	External Database	Details
Met -19	-	initiating methionine
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag

C, D: 845 - 893 (UniProt: O75113)

PDB	External Database	Details
Met 829	-	initiating methionine
His 830	-	expression tag
His 831	-	expression tag
His 832	-	expression tag
His 833	-	expression tag
His 834	-	expression tag
His 835	-	expression tag
Gly 836	-	expression tag
Glu 837	-	expression tag
Asn 838	-	expression tag
Leu 839	-	expression tag
Tyr 840	-	expression tag
Phe 841	-	expression tag
Gln 842	-	expression tag
Gly 843	-	expression tag
Ser 844	-	expression tag
Ser 852	Asn 855	variant
Lys 868	Arg 871	conflict
Ala 876	Val 879	conflict
Leu 890	Met 893	conflict
Gly 894	-	expression tag
Asn 895	-	expression tag
Ser 896	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	54079.4
Non-Polymers*	Number of molecules	2
	Total formula weight	99.0
All*	Total formula weight	54178.4

*Water molecules are not included.