8T3T

Structure of Bre1-nucleosome complex - state3

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, E (A, E)	Histone H3.2	polymer	135	15303.9	2	UniProt (P84233) Pfam (PF00125)	Xenopus laevis (African clawed frog)	Histone H3
2	B, F (B, F)	Histone H4	polymer	102	11263.2	2	UniProt (P62799) Pfam (PF15511)	Xenopus laevis (African clawed frog)
3	C, G (C, G)	Histone H2A type 1	polymer	129	13978.2	2	UniProt (P06897) Pfam (PF00125) Pfam (PF16211)	Xenopus laevis (African clawed frog)
4	D, H (D, H)	Histone H2B	polymer	122	13524.8	2	UniProt (P02281) Pfam (PF00125)	Xenopus laevis (African clawed frog)	H2B1.1
5	I (I)	601 DNA strand 1	polymer	146	44825.6	1		synthetic construct
6	J (J)	601 DNA strand 2	polymer	146	45305.9	1		synthetic construct
7	K, L (K, L)	E3 ubiquitin-protein ligase BRE1	polymer	110	12633.6	2	UniProt (Q07457) Pfam (PF00097)	Saccharomyces cerevisiae (baker's yeast)	Brefeldin A-sensitivity protein 1,RING-type E3 ubiquitin transferase BRE1
8	M, N, O, P (K, L)	ZINC ION	non-polymer		65.4	4	Chemie (ZN)

Sequence modifications

A, E: 1 - 135 (UniProt: P84233)

PDB	External Database	Details
Ala 102	Gly 103	variant

C, G: 1 - 129 (UniProt: P06897)

PDB	External Database	Details
Arg 99	Gly 100	conflict
Ser 123	Ala 124	conflict

D, H: 4 - 125 (UniProt: P02281)

PDB	External Database	Details
Thr 32	Ser 33	variant

Contents of the asymmetric unit

Polymers	Number of chains	12
	Total formula weight	223539.0
Non-Polymers*	Number of molecules	4
	Total formula weight	261.6
All*	Total formula weight	223800.7

*Water molecules are not included.