8T26

Crystal Structure of Porphyromonas gingivalis Sialidase (PG_0352) D219A mutant bound to 3'-Sialyllactose (only Neu5Ac visible)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Sialidase, putative	polymer	509	56494.3	1	UniProt (Q7MX62) Pfam (PF13088) In PDB	Porphyromonas gingivalis
2	A	N-acetyl-alpha-neuraminic acid	non-polymer		309.3	1	Chemie (SIA)
3	A	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	1	Chemie (PEG)
4	A	TRIETHYLENE GLYCOL	non-polymer		150.2	2	Chemie (PGE)
5	A	beta-D-galactopyranose	non-polymer		180.2	1	Chemie (GAL)
6		water	water		18.0	489	Chemie (HOH)

Sequence modifications

A: 31 - 526 (UniProt: Q7MX62)

PDB	External Database	Details
Met 18	-	expression tag
Arg 19	-	expression tag
Gly 20	-	expression tag
Ser 21	-	expression tag
His 22	-	expression tag
His 23	-	expression tag
His 24	-	expression tag
His 25	-	expression tag
His 26	-	expression tag
His 27	-	expression tag
Gly 28	-	expression tag
Ser 29	-	expression tag
Met 30	-	expression tag
Ala 219	Asp 219	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	56494.3
Non-Polymers*	Number of molecules	5
	Total formula weight	895.9
All*	Total formula weight	57390.2

*Water molecules are not included.