8T1F

Cryo-EM structure of full-length human TRPV4 in complex with antagonist HC-067047

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	Transient receptor potential cation channel subfamily V member 4/Enhanced green fluorescent protein chimera	polymer	1132	127717.9	4	UniProt (Q9HBA0) UniProt (C5MKY7) Pfam (PF00023) Pfam (PF00520)	Homo sapiens (human)	TrpV4,Osm-9-like TRP channel 4,OTRPC4,Transient receptor potential protein 12,TRP12,Vanilloid receptor-like channel 2,Vanilloid receptor-like protein 2,VRL-2,Vanilloid receptor-related osmotically-activated channel,VR-OAC
2	E, G, I, K (A, B, C, D)	2-methyl-1-[3-(morpholin-4-yl)propyl]-5-phenyl-N-[3-(trifluoromethyl)phenyl]-1H-pyrrole-3-carboxamide	non-polymer		471.5	4	Chemie (X7N)
3	F, H, J, L (A, B, C, D)	(2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate	non-polymer		760.1	4	Chemie (POV)

Sequence modifications

A, B, C, D: 1 - 871 (UniProt: Q9HBA0)

PDB	External Database	Details
Leu 872	-	linker
Val 873	-	linker
Pro 874	-	linker
Arg 875	-	linker
Gly 876	-	linker
Ser 877	-	linker
Ala 878	-	linker
Ala 879	-	linker
Ala 880	-	linker
Ala 881	-	linker

A, B, C, D: 882 - 1119 (UniProt: C5MKY7)

PDB	External Database	Details
Lys 1087	Ala 207	conflict
Ser 1120	-	expression tag
Gly 1121	-	expression tag
Leu 1122	-	expression tag
Arg 1123	-	expression tag
Ser 1124	-	expression tag
Trp 1125	-	expression tag
Ser 1126	-	expression tag
His 1127	-	expression tag
Pro 1128	-	expression tag
Gln 1129	-	expression tag
Phe 1130	-	expression tag
Glu 1131	-	expression tag
Lys 1132	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	510871.8
Non-Polymers*	Number of molecules	8
	Total formula weight	4926.4
All*	Total formula weight	515798.1

*Water molecules are not included.