8T1E
Closed-state cryo-EM structure of full-length human TRPV4 in the presence of 4a-PDD
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D (A, B, C, D) | Transient receptor potential cation channel subfamily V member 4,Enhanced green fluorescent protein | polymer | 1132 | 127717.9 | 4 | UniProt (Q9HBA0) UniProt (C5MKY7) Pfam (PF00023) Pfam (PF00520) | Homo sapiens (human) | TrpV4,Osm-9-like TRP channel 4,OTRPC4,Transient receptor potential protein 12,TRP12,Vanilloid receptor-like channel 2,Vanilloid receptor-like protein 2,VRL-2,Vanilloid receptor-related osmotically-activated channel,VR-OAC |
| 2 | AA, BA, DA, E, EA... (B, C, A, D) | [(2~{R})-2-[(~{Z})-hexadec-9-enoyl]oxy-3-[oxidanyl-[2-(trimethyl-$l^{4}-azanyl)ethoxy]phosphoryl]oxy-propyl] (~{Z})-docos-13-enoate | non-polymer | 815.2 | 32 | Chemie (9ZR) | |||
| 3 | CA, JA, K, N, NA... (C, A, D, B) | (2R)-2-{[(4-O-hexopyranosyl-beta-D-glucopyranosyl)oxy]methyl}-4-{[(25R)-5beta,14beta,17beta-spirostan-3beta-yl]oxy}butyl 4-O-alpha-D-glucopyranosyl-beta-D-glucopyranoside | non-polymer | 1165.3 | 8 | Chemie (YJ0) | |||
| 4 | O, P, Q (A) | SODIUM ION | non-polymer | 23.0 | 3 | Chemie (NA) | |||
| 5 | VA, WA, XA, YA (A, B, C, D) | water | water | 18.0 | 36 | Chemie (HOH) |
Sequence modifications
A, B, C, D: 1 - 871 (UniProt: Q9HBA0)
A, B, C, D: 882 - 1119 (UniProt: C5MKY7)
| PDB | External Database | Details |
|---|---|---|
| Leu 872 | - | linker |
| Val 873 | - | linker |
| Pro 874 | - | linker |
| Arg 875 | - | linker |
| Gly 876 | - | linker |
| Ser 877 | - | linker |
| Ala 878 | - | linker |
| Ala 879 | - | linker |
| Ala 880 | - | linker |
| Ala 881 | - | linker |
| PDB | External Database | Details |
|---|---|---|
| Lys 1087 | Ala 207 | engineered mutation |
| Ser 1120 | - | expression tag |
| Gly 1121 | - | expression tag |
| Leu 1122 | - | expression tag |
| Arg 1123 | - | expression tag |
| Ser 1124 | - | expression tag |
| Trp 1125 | - | expression tag |
| Ser 1126 | - | expression tag |
| His 1127 | - | expression tag |
| Pro 1128 | - | expression tag |
| Gln 1129 | - | expression tag |
| Phe 1130 | - | expression tag |
| Glu 1131 | - | expression tag |
| Lys 1132 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 510871.8 | |
| Non-Polymers* | Number of molecules | 43 |
| Total formula weight | 35477.1 | |
| All* | Total formula weight | 546348.8 |
