8T1D

Open-state cryo-EM structure of full-length human TRPV4 in complex with agonist 4a-PDD

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D	Transient receptor potential cation channel subfamily V member 4/Enhanced green fluorescent protein chimera	polymer	1132	127717.9	4	UniProt (Q9HBA0) UniProt (C5MKY7) Pfam (PF00023) Pfam (PF00520) In PDB	Homo sapiens (human)	TrpV4,Osm-9-like TRP channel 4,OTRPC4,Transient receptor potential protein 12,TRP12,Vanilloid receptor-like channel 2,Vanilloid receptor-like protein 2,VRL-2,Vanilloid receptor-related osmotically-activated channel,VR-OAC
2	A, B, C, D	(1aR,1bS,4aS,7aS,7bS,8R,9R,9aS)-9a-(decanoyloxy)-4a,7b-dihydroxy-3-(hydroxymethyl)-1,1,6,8-tetramethyl-5-oxo-1a,1b,4,4a,5,7a,7b,8,9,9a-decahydro-1H-cyclopropa[3,4]benzo[1,2-e]azulen-9-yl decanoate	non-polymer		672.9	4	Chemie (XS9)

Sequence modifications

A, B, C, D: 1 - 871 (UniProt: Q9HBA0)

PDB	External Database	Details
Leu 872	-	linker
Val 873	-	linker
Pro 874	-	linker
Arg 875	-	linker
Gly 876	-	linker
Ser 877	-	linker
Ala 878	-	linker
Ala 879	-	linker
Ala 880	-	linker
Ala 881	-	linker

A, B, C, D: 882 - 1119 (UniProt: C5MKY7)

PDB	External Database	Details
Lys 1087	Ala 207	engineered mutation
Ser 1120	-	expression tag
Gly 1121	-	expression tag
Leu 1122	-	expression tag
Arg 1123	-	expression tag
Ser 1124	-	expression tag
Trp 1125	-	expression tag
Ser 1126	-	expression tag
His 1127	-	expression tag
Pro 1128	-	expression tag
Gln 1129	-	expression tag
Phe 1130	-	expression tag
Glu 1131	-	expression tag
Lys 1132	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	510871.8
Non-Polymers*	Number of molecules	4
	Total formula weight	2691.7
All*	Total formula weight	513563.5

*Water molecules are not included.