8T18

Cryo-EM structure of dodecameric hub domain of CaMKII beta

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E... (A, B, C, D, E...)	Venus-tagged CaMKII Alpha Association Domain	polymer	407	45629.1	12	UniProt (P42212) UniProt (P08413) Pfam (PF01353) Pfam (PF08332)	Aequorea victoria

Sequence modifications

A, B, C, D, E, F, G, H, I, J, K, L: 157 - 393 (UniProt: P42212)

PDB	External Database	Details
Met 136	-	initiating methionine
Gly 137	-	expression tag
Ser 138	-	expression tag
Ser 139	-	expression tag
His 140	-	expression tag
His 141	-	expression tag
His 142	-	expression tag
His 143	-	expression tag
His 144	-	expression tag
His 145	-	expression tag
Ser 146	-	expression tag
Ser 147	-	expression tag
Gly 148	-	expression tag
Leu 149	-	expression tag
Val 150	-	expression tag
Pro 151	-	expression tag
Arg 152	-	expression tag
Gly 153	-	expression tag
Ser 154	-	expression tag
His 155	-	expression tag
Val 156	-	expression tag
Leu 201	Phe 46	conflict
Leu 219	Phe 64	conflict
Gly 220	Ser 65	conflict
Leu 223	Val 68	conflict
Ala 227	Ser 72	conflict
Thr 308	Met 153	conflict
Ala 318	Val 163	conflict
Gly 330	Ser 175	conflict
Tyr 358	Thr 203	conflict
Lys 361	Ala 206	conflict
Leu 386	His 231	conflict
Ser 394	-	linker
Gly 395	-	linker
Leu 396	-	linker
Arg 397	-	linker
Ser 398	-	linker
Arg 399	-	linker
Ala 400	-	linker
Gln 401	-	linker
Ala 402	-	linker
Ser 403	-	linker
Asn 404	-	linker
Ser 405	-	linker
Ala 406	-	linker
Val 407	-	linker
Asp 408	-	linker

A, B, C, D, E, F, G, H, I, J, K, L: 409 - 542 (UniProt: P08413)

Contents of the asymmetric unit

Polymers	Number of chains	12
	Total formula weight	547549.7
All*	Total formula weight	547549.7

*Water molecules are not included.