8T0L
E. coli Sw2/Snf2 ATPase RapA bound to both ADP-AlF3 and reconstituted E. coli RNA polymerase post-termination complex on negatively-supercoiled DNA
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (G, H) | DNA-directed RNA polymerase subunit alpha | polymer | 232 | 25597.1 | 2 | UniProt (C3SR67) Pfam (PF01193) Pfam (PF01000) UniProt (by SIFTS) (P0A7Z4) | Escherichia coli | RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha |
| 2 | C (I) | DNA-directed RNA polymerase subunit beta | polymer | 1340 | 150560.6 | 1 | UniProt (C3SIA7) Pfam (PF04561) Pfam (PF04565) Pfam (PF10385) Pfam (PF00562) Pfam (PF04560) Pfam (PF04563) UniProt (by SIFTS) (P0A8V2) | Escherichia coli | |
| 3 | D (J) | DNA-directed RNA polymerase subunit beta' | polymer | 1358 | 150406.3 | 1 | UniProt (A0A369F490) Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) UniProt (by SIFTS) (P0A8T7) | Escherichia coli | RNAP subunit beta',RNA polymerase subunit beta',Transcriptase subunit beta' |
| 4 | E (K) | DNA-directed RNA polymerase subunit omega | polymer | 72 | 8250.3 | 1 | UniProt (A7ZTK1) Pfam (PF01192) UniProt (by SIFTS) (P0A800) | Escherichia coli | RNAP omega subunit,RNA polymerase omega subunit,Transcriptase subunit omega |
| 5 | F (F) | RNA polymerase-associated protein RapA | polymer | 966 | 109096.9 | 1 | UniProt (B7MAI2) Pfam (PF18339) Pfam (PF18337) Pfam (PF00176) Pfam (PF00271) Pfam (PF12137) UniProt (by SIFTS) (P60240) | Escherichia coli | ATP-dependent helicase HepA |
| 6 | G (A) | DNA (29-MER) | polymer | 23 | 7158.8 | 1 | synthetic construct | ||
| 7 | H (B) | DNA (5'-D(P*TP*CP*TP*GP*AP*AP*TP*TP*TP*AP*AP*AP*TP*TP*CP*AP*GP*A)-3') | polymer | 23 | 6951.5 | 1 | synthetic construct | ||
| 8 | I (J) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) | |||
| 9 | J, K (J) | ZINC ION | non-polymer | 65.4 | 2 | Chemie (ZN) | |||
| 10 | L (F) | ADENOSINE-5'-DIPHOSPHATE | non-polymer | 427.2 | 1 | Chemie (ADP) | |||
| 11 | M (F) | ALUMINUM FLUORIDE | non-polymer | 84.0 | 1 | Chemie (AF3) |
Sequence modifications
G, H: 4 - 234 (UniProt: C3SR67)
J: 16 - 1373 (UniProt: A0A369F490)
F: 2 - 967 (UniProt: B7MAI2)
| PDB | External Database | Details |
|---|---|---|
| Ala 235 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Ala 262 | Thr 262 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Ala 144 | Tyr 144 | conflict |
| Ala 413 | Thr 413 | conflict |
| Ala 428 | Phe 428 | conflict |
| Ala 596 | Arg 596 | conflict |
| Ala 640 | Arg 640 | conflict |
| Ala 685 | Arg 685 | conflict |
| Ala 714 | Asn 714 | conflict |
| Ala 725 | Ile 725 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 8 |
| Total formula weight | 483618.6 | |
| Non-Polymers* | Number of molecules | 5 |
| Total formula weight | 666.3 | |
| All* | Total formula weight | 484284.9 |
