8T00
Reconstituted E. coli RNA polymerase post-termination complex on negatively-supercoiled DNA: closed duplex DNA (rPTCc)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (G, H) | DNA-directed RNA polymerase subunit alpha | polymer | 234 | 25897.5 | 2 | UniProt (A0A5B9AW69) Pfam (PF01193) Pfam (PF01000) UniProt (by SIFTS) (P0A7Z4) | Escherichia coli | RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha |
| 2 | C (I) | DNA-directed RNA polymerase subunit beta | polymer | 1340 | 150560.6 | 1 | UniProt (C3SIA7) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF10385) Pfam (PF00562) Pfam (PF04560) UniProt (by SIFTS) (P0A8V2) | Escherichia coli | |
| 3 | D (J) | DNA-directed RNA polymerase subunit beta' | polymer | 1358 | 150350.2 | 1 | UniProt (A0A369F490) Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) UniProt (by SIFTS) (P0A8T7) | Escherichia coli | RNAP subunit beta',RNA polymerase subunit beta',Transcriptase subunit beta' |
| 4 | E (A) | DNA (26-MER) | polymer | 26 | 8098.4 | 1 | synthetic construct | ||
| 5 | F (B) | DNA (26-MER) | polymer | 26 | 7864.1 | 1 | synthetic construct | ||
| 6 | G (J) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) | |||
| 7 | H, I (J) | ZINC ION | non-polymer | 65.4 | 2 | Chemie (ZN) |
Sequence modifications
J: 16 - 1373 (UniProt: A0A369F490)
| PDB | External Database | Details |
|---|---|---|
| Ala 1369 | Arg 1369 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 6 |
| Total formula weight | 368668.2 | |
| Non-Polymers* | Number of molecules | 3 |
| Total formula weight | 155.1 | |
| All* | Total formula weight | 368823.3 |
