8SYD
X-ray crystal structure of UDP-2,3-diacetamido-2,3-dideoxy-glucuronic acid-2-epimerase from Thermus thermophilus strain HB27, D98N variant in the presence of UDP-2,3-diacetamido-2,3-dideoxy-glucuronic acid and UDP-N-acetylglucosamine at pH 6
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, B | UDP-2,3-diacetamido-2,3-dideoxy-glucuronic acid-2-epimerase | polymer | 385 | 43278.4 | 2 | UniProt (Q72KY0) Pfam (PF02350) In PDB | Thermus thermophilus HB27 | |
2 | A, B | (2~{S},3~{S},4~{R},5~{R},6~{R})-4,5-diacetamido-6-[[[(2~{R},3~{S},4~{R},5~{R})-5-[2,4-bis(oxidanylidene)pyrimidin-1-yl]-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-3-oxidanyl-oxane-2-carboxylic acid | non-polymer | 662.4 | 2 | Chemie (MJL) | |||
3 | A, B | URIDINE-DIPHOSPHATE-N-ACETYLGLUCOSAMINE | non-polymer | 607.4 | 2 | Chemie (UD1) | |||
4 | A, B | CHLORIDE ION | non-polymer | 35.5 | 2 | Chemie (CL) | |||
5 | water | water | 18.0 | 274 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 365 (UniProt: Q72KY0)
PDB | External Database | Details |
---|---|---|
Met -19 | - | initiating methionine |
Gly -18 | - | expression tag |
Ser -17 | - | expression tag |
Ser -16 | - | expression tag |
His -15 | - | expression tag |
His -14 | - | expression tag |
His -13 | - | expression tag |
His -12 | - | expression tag |
His -11 | - | expression tag |
His -10 | - | expression tag |
Ser -9 | - | expression tag |
Ser -8 | - | expression tag |
Glu -7 | - | expression tag |
Asn -6 | - | expression tag |
Leu -5 | - | expression tag |
Tyr -4 | - | expression tag |
Phe -3 | - | expression tag |
Gln -2 | - | expression tag |
Gly -1 | - | expression tag |
His 0 | - | expression tag |
Asn 98 | Asp 98 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 2 |
Total formula weight | 86556.8 | |
Non-Polymers* | Number of molecules | 6 |
Total formula weight | 2610.4 | |
All* | Total formula weight | 89167.2 |