8SYB

X-ray crystal structure of UDP-2,3-diacetamido-2,3-dideoxy-glucuronic acid-2-epimerase from Thermus thermophilus strain HB27, D98N variant in the presence of UDP-2,3-diacetamido-2,3-dideoxy-glucuronic acid and UDP-N-acetylglucosamine at pH 9

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	UDP-2,3-diacetamido-2,3-dideoxy-glucuronic acid-2-epimerase	polymer	373	41994.1	2	UniProt (Q72KY0) Pfam (PF02350) In PDB	Thermus thermophilus HB27
2	A, B	(2~{S},3~{S},4~{R},5~{R},6~{R})-4,5-diacetamido-6-[[[(2~{R},3~{S},4~{R},5~{R})-5-[2,4-bis(oxidanylidene)pyrimidin-1-yl]-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-3-oxidanyl-oxane-2-carboxylic acid	non-polymer		662.4	2	Chemie (MJL)
3	A, B	URIDINE-DIPHOSPHATE-N-ACETYLGLUCOSAMINE	non-polymer		607.4	2	Chemie (UD1)
4	A, B	CHLORIDE ION	non-polymer		35.5	2	Chemie (CL)
5	B	SODIUM ION	non-polymer		23.0	1	Chemie (NA)
6		water	water		18.0	226	Chemie (HOH)

Sequence modifications

A, B: 1 - 365 (UniProt: Q72KY0)

PDB	External Database	Details
Asn 98	Asp 98	engineered mutation
Leu 366	-	expression tag
Glu 367	-	expression tag
His 368	-	expression tag
His 369	-	expression tag
His 370	-	expression tag
His 371	-	expression tag
His 372	-	expression tag
His 373	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	83988.2
Non-Polymers*	Number of molecules	7
	Total formula weight	2633.4
All*	Total formula weight	86621.6

*Water molecules are not included.