8SYA

X-ray crystal structure of UDP-2,3-diacetamido-2,3-dideoxy-glucuronic acid-2-epimerase from Thermus thermophilus strain HB27, D98N variant in the presence of UDP-2,3-diacetamido-2,3-dideoxy-glucuronic acid and UDP at pH 9

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	UDP-2,3-diacetamido-2,3-dideoxy-glucuronic acid-2-epimerase	polymer	373	41994.1	2	UniProt (Q72KY0) Pfam (PF02350)	Thermus thermophilus HB27
2	C, D (A, B)	(2~{S},3~{S},4~{R},5~{R},6~{R})-4,5-diacetamido-6-[[[(2~{R},3~{S},4~{R},5~{R})-5-[2,4-bis(oxidanylidene)pyrimidin-1-yl]-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-3-oxidanyl-oxane-2-carboxylic acid	non-polymer		662.4	2	Chemie (MJL)
3	E (B)	URIDINE-5'-DIPHOSPHATE	non-polymer		404.2	1	Chemie (UDP)
4	F, G (A, B)	water	water		18.0	151	Chemie (HOH)

Sequence modifications

A, B: 1 - 365 (UniProt: Q72KY0)

PDB	External Database	Details
Asn 98	Asp 98	engineered mutation
Leu 366	-	expression tag
Glu 367	-	expression tag
His 368	-	expression tag
His 369	-	expression tag
His 370	-	expression tag
His 371	-	expression tag
His 372	-	expression tag
His 373	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	83988.2
Non-Polymers*	Number of molecules	3
	Total formula weight	1728.9
All*	Total formula weight	85717.1

*Water molecules are not included.