8SWC

RNase H complex with ASO (OOO) and RNA

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Ribonuclease H1	polymer	294	33218.5	1	UniProt (O60930) Pfam (PF01693) Pfam (PF00075)	Homo sapiens (human)	RNase H1,Ribonuclease H type II
2	B (B)	RNA (5'-R(*UP*GP*GP*CP*GP*AP*GP*UP*GP*GP*GP*UP*GP*AP*GP*UP*GP*AP*GP*G)-3')	polymer	20	6615.0	1		Homo sapiens
3	C (C)	PO ASO	polymer	20	6380.3	1		Homo sapiens
4	D, G (A, B)	PHOSPHATE ION	non-polymer		95.0	2	Chemie (PO4)
5	E, H, I (A, B)	CALCIUM ION	non-polymer		40.1	3	Chemie (CA)
6	F (B)	SULFATE ION	non-polymer		96.1	1	Chemie (SO4)
7	J, K, L (A, B, C)	water	water		18.0	5	Chemie (HOH)

Sequence modifications

A: 1 - 286 (UniProt: O60930)

PDB	External Database	Details
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
Asn 210	Asp 210	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	46213.7
Non-Polymers*	Number of molecules	6
	Total formula weight	406.2
All*	Total formula weight	46620.0

*Water molecules are not included.