8SWA

Crystal structure of the human S-adenosylmethionine synthetase 1 in complex with SAM and PPNP

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	S-adenosylmethionine synthase isoform type-1	polymer	403	44837.1	1	UniProt (Q00266) Pfam (PF00438) Pfam (PF02772) Pfam (PF02773) In PDB	Homo sapiens (human)	AdoMet synthase 1,Methionine adenosyltransferase 1,MAT 1,Methionine adenosyltransferase I/III,MAT-I/III
2	A	(DIPHOSPHONO)AMINOPHOSPHONIC ACID	non-polymer		257.0	1	Chemie (PPK)
3	A	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
4	A	POTASSIUM ION	non-polymer		39.1	1	Chemie (K)
5	A	S-ADENOSYLMETHIONINE	non-polymer		398.4	1	Chemie (SAM)
6		water	water		18.0	231	Chemie (HOH)

Sequence modifications

A: 16 - 395 (UniProt: Q00266)

PDB	External Database	Details
Met -7	-	initiating methionine
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
Ser 0	-	expression tag
Ser 1	-	expression tag
Gly 2	-	expression tag
Val 3	-	expression tag
Asp 4	-	expression tag
Leu 5	-	expression tag
Gly 6	-	expression tag
Thr 7	-	expression tag
Glu 8	-	expression tag
Asn 9	-	expression tag
Leu 10	-	expression tag
Tyr 11	-	expression tag
Phe 12	-	expression tag
Gln 13	-	expression tag
Ser 14	-	expression tag
Met 15	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	44837.1
Non-Polymers*	Number of molecules	5
	Total formula weight	743.1
All*	Total formula weight	45580.2

*Water molecules are not included.