8SVF

BAP1/ASXL1 bound to the H2AK119Ub Nucleosome

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, E (A, E)	Histone H3.2	polymer	136	15435.1	2	UniProt (P84233) Pfam (PF00125)	Xenopus laevis (African clawed frog)	Histone H3
2	B, F (B, F)	Histone H4	polymer	103	11394.4	2	UniProt (P62799) Pfam (PF15511)	Xenopus laevis (African clawed frog)
3	C, G (C, G)	Histone H2A type 1	polymer	130	14093.4	2	UniProt (P06897) Pfam (PF00125) Pfam (PF16211)	Xenopus laevis (African clawed frog)
4	D, H (D, H)	Histone H2B 1.1	polymer	126	13979.3	2	UniProt (P02281) Pfam (PF00125)	Xenopus laevis (African clawed frog)	H2B1.1
5	I (I)	DNA/RNA (187-MER)	polymer	187	58568.8	1		synthetic construct
6	J (J)	DNA/RNA (327-MER)	polymer	327	102866.5	1		synthetic construct
7	K (K)	Ubiquitin carboxyl-terminal hydrolase BAP1	polymer	729	80472.6	1	UniProt (Q92560) Pfam (PF01088) Pfam (PF18031)	Homo sapiens (human)	BRCA1-associated protein 1,Cerebral protein 6
8	L (L)	Polycomb group protein ASXL1	polymer	1541	165635.2	1	UniProt (Q8IXJ9) Pfam (PF13922) Pfam (PF05066) Pfam (PF13919)	Homo sapiens	Additional sex combs-like protein 1
9	M (M)	Ubiquitin	polymer	76	8576.8	1	UniProt (P0CG48) Pfam (PF00240)	Homo sapiens (human)

Sequence modifications

A, E: 0 - 135 (UniProt: P84233)

PDB	External Database	Details
Ala 102	Gly 103	engineered mutation

C, G: 0 - 129 (UniProt: P06897)

PDB	External Database	Details
Arg 99	Gly 100	engineered mutation

D, H: -3 - 122 (UniProt: P02281)

PDB	External Database	Details
Thr 29	Ser 33	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	13
	Total formula weight	525924.5
All*	Total formula weight	525924.5

*Water molecules are not included.