8SV3
7-Deazapurines and 5-Halogenpyrimidine DNA duplex
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C (A, B, C) | Ribonuclease H | polymer | 142 | 16329.5 | 3 | UniProt (Q9KEI9) | Halalkalibacterium halodurans | RNase H |
| 2 | D, E (D, E) | Modified DNA | polymer | 12 | 3690.3 | 2 | synthetic construct | ||
| 3 | F (A) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) | |||
| 4 | G, H (B, C) | SODIUM ION | non-polymer | 23.0 | 2 | Chemie (NA) | |||
| 5 | I (D) | GLYCEROL | non-polymer | 92.1 | 1 | Chemie (GOL) | |||
| 6 | J, K, L, M, N (A, B, C, D, E) | water | water | 18.0 | 492 | Chemie (HOH) |
Sequence modifications
A, B, C: 59 - 196 (UniProt: Q9KEI9)
| PDB | External Database | Details |
|---|---|---|
| Gly 55 | - | expression tag |
| Ser 56 | - | expression tag |
| His 57 | - | expression tag |
| Met 58 | - | expression tag |
| Asn 132 | Asp 132 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 5 |
| Total formula weight | 56369.0 | |
| Non-Polymers* | Number of molecules | 4 |
| Total formula weight | 162.4 | |
| All* | Total formula weight | 56531.4 |
