8ST7

Structure of E3 ligase VsHECT bound to ubiquitin

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (D, B)	Ubiquitin	polymer	75	8519.8	2	UniProt (P0CG48) Pfam (PF00240)	Homo sapiens (human)
2	C, D (A, C)	E3 ubiquitin-protein ligase SopA-like catalytic domain-containing protein	polymer	226	26111.7	2	UniProt (A0A2V2RSR1) Pfam (PF13979)	Verrucomicrobiota
3	E, F (D, B)	prop-2-en-1-amine	non-polymer		57.1	2	Chemie (AYE)
4	G, H, I, J (D, B, A, C)	water	water		18.0	540	Chemie (HOH)

Sequence modifications

A, C: 639 - 847 (UniProt: A0A2V2RSR1)

PDB	External Database	Details
His 622	-	expression tag
His 623	-	expression tag
His 624	-	expression tag
His 625	-	expression tag
His 626	-	expression tag
His 627	-	expression tag
Ser 628	-	expression tag
Ser 629	-	expression tag
Gly 630	-	expression tag
Leu 631	-	expression tag
Glu 632	-	expression tag
Val 633	-	expression tag
Leu 634	-	expression tag
Phe 635	-	expression tag
Gln 636	-	expression tag
Gly 637	-	expression tag
Pro 638	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	69263.0
Non-Polymers*	Number of molecules	2
	Total formula weight	114.2
All*	Total formula weight	69377.1

*Water molecules are not included.