8SM8

Air-oxidized A. ca TruffO expressed from M9 minimal medium supplemented with Fe

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	G, A, D, J	Putative amidohydrolase	polymer	388	43894.6	4	UniProt (B6JH23) Pfam (PF04909) In PDB	Afipia carboxidovorans OM5
2	G, A, D, J	FE (III) ION	non-polymer		55.8	8	Chemie (FE)
3	G, A, D, J	SULFATE ION	non-polymer		96.1	4	Chemie (SO4)
4	A	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
5		water	water		18.0	736	Chemie (HOH)

Sequence modifications

G, A, D, J: 1 - 357 (UniProt: B6JH23)

PDB	External Database	Details
Met -30	-	initiating methionine
Gly -29	-	expression tag
Ser -28	-	expression tag
Ser -27	-	expression tag
His -26	-	expression tag
His -25	-	expression tag
His -24	-	expression tag
His -23	-	expression tag
His -22	-	expression tag
His -21	-	expression tag
Ser -20	-	expression tag
Ser -19	-	expression tag
Gly -18	-	expression tag
Leu -17	-	expression tag
Val -16	-	expression tag
Pro -15	-	expression tag
Arg -14	-	expression tag
Gly -13	-	expression tag
Ser -12	-	expression tag
His -11	-	expression tag
Met -10	-	expression tag
Gly -9	-	expression tag
Glu -8	-	expression tag
Asn -7	-	expression tag
Leu -6	-	expression tag
Tyr -5	-	expression tag
Phe -4	-	expression tag
Gln -3	-	expression tag
Ser -2	-	expression tag
Gly -1	-	expression tag
Gly 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	175578.2
Non-Polymers*	Number of molecules	14
	Total formula weight	879.6
All*	Total formula weight	176457.9

*Water molecules are not included.