8SLC

High Affinity nanobodies against GFP

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Green fluorescent protein	polymer	255	28794.4	2	UniProt (P42212) Pfam (PF01353)	Aequorea victoria
2	C, D (C, D)	LaG43	polymer	148	16186.0	2		Lama glama
3	E, H, I, J (A, B, C)	GLYCEROL	non-polymer		92.1	4	Chemie (GOL)
4	F, K, L, M (A, C, D)	CHLORIDE ION	non-polymer		35.5	4	Chemie (CL)
5	G (A)	SODIUM ION	non-polymer		23.0	1	Chemie (NA)
6	N, O, P, Q (A, B, C, D)	water	water		18.0	9	Chemie (HOH)

Sequence modifications

A, B: 2 - 238 (UniProt: P42212)

PDB	External Database	Details
Met 0	-	initiating methionine
Ala 1	-	expression tag
Cr2 66	Ser 65	chromophore
Cr2 66	Tyr 66	chromophore
Cr2 66	Gly 67	chromophore
Ala 72	Ser 72	conflict
His 177	Gln 177	conflict
Gly 239	-	expression tag
Leu 240	-	expression tag
Glu 241	-	expression tag
Val 242	-	expression tag
Leu 243	-	expression tag
Phe 244	-	expression tag
Gln 245	-	expression tag
Gly 246	-	expression tag
Pro 247	-	expression tag
Ser 248	-	expression tag
His 249	-	expression tag
His 250	-	expression tag
His 251	-	expression tag
His 252	-	expression tag
His 253	-	expression tag
His 254	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	89960.8
Non-Polymers*	Number of molecules	9
	Total formula weight	533.2
All*	Total formula weight	90493.9

*Water molecules are not included.