8SKZ
Cryo-EM structure of DDM1-HELLS chimera bound to the nucleosome
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | ATP-dependent DNA helicase DDM1,Lymphoid-specific helicase chimera | polymer | 821 | 93326.2 | 1 | UniProt (Q9XFH4) UniProt (Q9NRZ9) Pfam (PF00176) Pfam (PF00271) | Arabidopsis thaliana (thale cress) | |
| 2 | B, E (B, E) | Histone H3.2 | polymer | 136 | 15435.1 | 2 | UniProt (P84233) Pfam (PF00125) | Xenopus laevis (African clawed frog) | Histone H3 |
| 3 | C, G (C, G) | Histone H2A | polymer | 133 | 14381.7 | 2 | UniProt (Q6AZJ8) Pfam (PF00125) Pfam (PF16211) UniProt (by SIFTS) (P06897) | Xenopus laevis (African clawed frog) | |
| 4 | D, H (D, H) | Histone H2B 1.1 | polymer | 123 | 13655.9 | 2 | UniProt (P02281) Pfam (PF00125) | Xenopus laevis (African clawed frog) | H2B1.1 |
| 5 | F, I (F, I) | Histone H4 | polymer | 110 | 12276.4 | 2 | UniProt (A0A8J1LTD2) Pfam (PF15511) | Xenopus laevis (African clawed frog) | |
| 6 | J (J) | DNA (192-MER) | polymer | 192 | 59004.6 | 1 | Homo sapiens | ||
| 7 | K (K) | DNA (192-MER) | polymer | 192 | 59546.9 | 1 | Homo sapiens | ||
| 8 | L (A) | ADENOSINE-5'-DIPHOSPHATE | non-polymer | 427.2 | 1 | Chemie (ADP) | |||
| 9 | M (A) | BERYLLIUM TRIFLUORIDE ION | non-polymer | 66.0 | 1 | Chemie (BEF) |
Sequence modifications
A: 1 - 479 (UniProt: Q9XFH4)
A: 480 - 533 (UniProt: Q9NRZ9)
A: 534 - 818 (UniProt: Q9XFH4)
B, E: 0 - 135 (UniProt: P84233)
C, G: 0 - 129 (UniProt: Q6AZJ8)
D, H: 1 - 122 (UniProt: P02281)
F, I: 0 - 102 (UniProt: A0A8J1LTD2)
| PDB | External Database | Details |
|---|---|---|
| Ser -2 | - | expression tag |
| Asn -1 | - | expression tag |
| Ala 0 | - | expression tag |
A: 534 - 818 (UniProt: Q9XFH4)
B, E: 0 - 135 (UniProt: P84233)
| PDB | External Database | Details |
|---|---|---|
| Ala 102 | Gly 103 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Ser -3 | - | expression tag |
| Asn -2 | - | expression tag |
| Ala -1 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Met 0 | - | initiating methionine |
| Thr 29 | Ser 33 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Ser 103 | - | expression tag |
| Glu 104 | - | expression tag |
| Asn 105 | - | expression tag |
| Leu 106 | - | expression tag |
| Tyr 107 | - | expression tag |
| Phe 108 | - | expression tag |
| Gln 109 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 11 |
| Total formula weight | 323376.0 | |
| Non-Polymers* | Number of molecules | 2 |
| Total formula weight | 493.2 | |
| All* | Total formula weight | 323869.2 |
