8SFZ

High Affinity nanobodies against GFP

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C (C, A, B)	LaG35	polymer	145	16028.7	3		Lama glama
2	D, F (D, F)	Green fluorescent protein	polymer	255	28794.4	2	UniProt (P42212) Pfam (PF01353)	Aequorea victoria
3	E (E)	Green fluorescent protein	polymer	255	28838.5	1	UniProt (P42212) Pfam (PF01353)	Aequorea victoria
4	G, H (C, A)	SODIUM ION	non-polymer		23.0	2	Chemie (NA)
5	I, J, K (B, D, F)	POTASSIUM ION	non-polymer		39.1	3	Chemie (K)
6	L, M, N, O, P... (C, A, B, D, E...)	water	water		18.0	406	Chemie (HOH)

Sequence modifications

D, F: 0 - 238 (UniProt: P42212)

PDB	External Database	Details
Ala 1	-	insertion
Cr2 66	Ser 65	chromophore
Cr2 66	Tyr 66	chromophore
Cr2 66	Gly 67	chromophore
Ala 72	Ser 72	conflict
His 177	Gln 177	conflict
Gly 239	-	expression tag
Leu 240	-	expression tag
Glu 241	-	expression tag
Val 242	-	expression tag
Leu 243	-	expression tag
Phe 244	-	expression tag
Gln 245	-	expression tag
Gly 246	-	expression tag
Pro 247	-	expression tag
Ser 248	-	expression tag
His 249	-	expression tag
His 250	-	expression tag
His 251	-	expression tag
His 252	-	expression tag
His 253	-	expression tag
His 254	-	expression tag

E: 0 - 239 (UniProt: P42212)

PDB	External Database	Details
Ala 1	-	insertion
Cro 66	Ser 65	chromophore
Cro 66	Tyr 66	chromophore
Cro 66	Gly 67	chromophore
Ala 72	Ser 72	conflict
His 177	Gln 177	conflict
Gly 240	-	expression tag
Leu 241	-	expression tag
Glu 242	-	expression tag
Val 243	-	expression tag
Leu 244	-	expression tag
Phe 245	-	expression tag
Gln 246	-	expression tag
Gly 247	-	expression tag
Pro 248	-	expression tag
Ser 249	-	expression tag
His 250	-	expression tag
His 251	-	expression tag
His 252	-	expression tag
His 253	-	expression tag
His 254	-	expression tag
His 255	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	134513.3
Non-Polymers*	Number of molecules	5
	Total formula weight	163.3
All*	Total formula weight	134676.6

*Water molecules are not included.