8SFX

High Affinity nanobodies against GFP

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Green fluorescent protein	polymer	255	28794.4	2	UniProt (P42212) Pfam (PF01353)	Aequorea victoria
2	C, D (D, C)	LaG21	polymer	142	15470.2	2		Lama glama
3	E, F, GA, N, O (A, C, B)	GLYCEROL	non-polymer		92.1	5	Chemie (GOL)
4	G, P (A, B)	D-MALATE	non-polymer		134.1	2	Chemie (MLT)
5	CA, DA, H, HA, I... (D, A, C, B)	SODIUM ION	non-polymer		23.0	19	Chemie (NA)
6	AA, BA, EA, FA, KA... (B, D, C, A)	PHOSPHATE ION	non-polymer		95.0	7	Chemie (PO4)
7	LA, MA, NA, OA (A, B, D, C)	water	water		18.0	146	Chemie (HOH)

Sequence modifications

A, B: 0 - 238 (UniProt: P42212)

PDB	External Database	Details
Ala 1	-	insertion
Cr2 66	Ser 65	chromophore
Cr2 66	Tyr 66	chromophore
Cr2 66	Gly 67	chromophore
Ala 72	Ser 72	conflict
His 177	Gln 177	conflict
Gly 239	-	expression tag
Leu 240	-	expression tag
Glu 241	-	expression tag
Val 242	-	expression tag
Leu 243	-	expression tag
Phe 244	-	expression tag
Gln 245	-	expression tag
Gly 246	-	expression tag
Pro 247	-	expression tag
Ser 248	-	expression tag
His 249	-	expression tag
His 250	-	expression tag
His 251	-	expression tag
His 252	-	expression tag
His 253	-	expression tag
His 254	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	88529.2
Non-Polymers*	Number of molecules	33
	Total formula weight	1830.3
All*	Total formula weight	90359.4

*Water molecules are not included.