8SFJ

WT CRISPR-Cas12a with a 10bp R-loop

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	CRISPR-associated endonuclease Cas12a	polymer	1311	151705.2	1	UniProt (U2UMQ6) Pfam (PF18501) Pfam (PF21918) Pfam (PF18516) Pfam (PF18510) In PDB	Acidaminococcus sp. BV3L6	AsCpf1,CRISPR-associated endonuclease Cpf1
2	B	RNA (29-MER)	polymer	48	15352.0	1		synthetic construct
3	C	DNA (5'-D(P*CP*AP*CP*TP*TP*AP*TP*CP*AP*CP*TP*AP*AP*AP*AP*GP*AP*TP*CP*GP*GP*AP*AP*G)-3')	polymer	56	17211.1	1		synthetic construct
4	D	DNA (5'-D(P*CP*TP*TP*CP*CP*GP*AP*TP*CP*TP*TP*TP*TP*AP*GP*TP*GP*AP*T)-3')	polymer	56	17299.1	1		synthetic construct

Sequence modifications

A: 1 - 1307 (UniProt: U2UMQ6)

PDB	External Database	Details
Gly -3	-	expression tag
Ala -2	-	expression tag
Ala -1	-	expression tag
Ser 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	201567.4
All*	Total formula weight	201567.4

*Water molecules are not included.