8SE9
Cryo-EM structure of a double loaded human UBA7-UBE2L6-ISG15 thioester mimetic complex (Form 2)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Ubiquitin-like modifier-activating enzyme 7 | polymer | 1012 | 111822.1 | 1 | UniProt (P41226) Pfam (PF00899) Pfam (PF16190) Pfam (PF16191) Pfam (PF10585) Pfam (PF09358) | Homo sapiens (human) | Ubiquitin-activating enzyme 7,D8,Ubiquitin-activating enzyme E1 homolog |
| 2 | B, D (B, D) | Ubiquitin-like protein ISG15 | polymer | 157 | 17163.7 | 2 | UniProt (P05161) Pfam (PF00240) | Homo sapiens (human) | Interferon-induced 15 kDa protein,Interferon-induced 17 kDa protein,IP17,Ubiquitin cross-reactive protein,hUCRP |
| 3 | C (C) | Ubiquitin/ISG15-conjugating enzyme E2 L6 | polymer | 152 | 17643.4 | 1 | UniProt (O14933) Pfam (PF00179) | Homo sapiens (human) | E2 ubiquitin-conjugating enzyme L6,Retinoic acid-induced gene B protein,RIG-B,UbcH8,Ubiquitin carrier protein L6,Ubiquitin-protein ligase L6 |
| 4 | E (B) | ADENOSINE MONOPHOSPHATE | non-polymer | 347.2 | 1 | Chemie (AMP) PubChem (16397049) PubChem (16760266) PubChem (40467049) PubChem (40469468) PubChem (44348067) PubChem (45358029) PubChem (46874558) PubChem (57358045) PubChem (57879893) PubChem (57879902) PubChem (122197642) PubChem (162290139) PubChem (162946266) PubChem (171699505) PubChem (40459109) PubChem (46875232) PubChem (177678969) PubChem (6083) PubChem (1549371) PubChem (92144436) PubChem (12358354) PubChem (224) PubChem (34768) PubChem (83862) PubChem (446048) PubChem (515384) PubChem (638042) PubChem (5316444) PubChem (5742864) PubChem (12163610) PubChem (12358355) |
Sequence modifications
C: 2 - 153 (UniProt: O14933)
| PDB | External Database | Details |
|---|---|---|
| Ser 98 | Cys 98 | engineered mutation |
| Ser 102 | Cys 102 | engineered mutation |
| Lys 121 | Leu 121 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 163792.9 | |
| Non-Polymers* | Number of molecules | 1 |
| Total formula weight | 347.2 | |
| All* | Total formula weight | 164140.1 |
