8SCI
Bst DNA polymerase I Large Fragment mutant F710Y/D598A with 3'-amino primer, dGTP, and calcium time-resolved 1h
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, D (A, D) | DNA polymerase I | polymer | 579 | 66073.8 | 2 | UniProt (D9N168) Pfam (PF22619) Pfam (PF00476) | Geobacillus stearothermophilus | |
| 2 | B, E (B, E) | DNA primer/product | polymer | 10 | 3069.0 | 2 | synthetic construct | ||
| 3 | C, F (C, F) | DNA template | polymer | 13 | 3936.6 | 2 | synthetic construct | ||
| 4 | G, K (A, D) | DIPHOSPHATE | non-polymer | 173.9 | 2 | Chemie (DPO) | |||
| 5 | H, L (A, D) | 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE | non-polymer | 507.2 | 2 | Chemie (DGT) | |||
| 6 | I, M (A, D) | CALCIUM ION | non-polymer | 40.1 | 2 | Chemie (CA) | |||
| 7 | J (A) | SULFATE ION | non-polymer | 96.1 | 1 | Chemie (SO4) | |||
| 8 | N, O, P, Q, R... (A, B, C, D, E...) | water | water | 18.0 | 107 | Chemie (HOH) |
Sequence modifications
A, D: 298 - 876 (UniProt: D9N168)
| PDB | External Database | Details |
|---|---|---|
| Tyr 710 | Phe 413 | engineered mutation |
| Val 713 | Pro 416 | variant |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 6 |
| Total formula weight | 146158.7 | |
| Non-Polymers* | Number of molecules | 7 |
| Total formula weight | 1538.5 | |
| All* | Total formula weight | 147697.2 |
