8SBU
Crystal structure of MBP fusion with HPPK from Methanocaldococcus jannaschii
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B | Maltose/maltodextrin-binding periplasmic protein,6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase | polymer | 618 | 68721.6 | 2 | UniProt (P0AEX9) UniProt (Q59028) Pfam (PF01547) Pfam (PF01973) In PDB | Escherichia coli K-12 | MMBP,Maltodextrin-binding protein,Maltose-binding protein,MBP,HPPK,2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase,6-hydroxymethyl-7,8-dihydropterin diphosphokinase,6-HMPDK,7,8-dihydro-6-hydroxymethylpterin diphosphokinase,8-dihydro-6-hydroxymethylpterin pyrophosphokinase,PPPK |
| 2 | C, D | alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose | branched | 342.3 | 2 | In PDB BIRD (PRD_900001) GlyTouCan (G07411ON) | |||
| 3 | water | water | 18.0 | 339 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 377 (UniProt: P0AEX9)
A, B: 378 - 618 (UniProt: Q59028)
| PDB | External Database | Details |
|---|---|---|
| - | Lys 4 | deletion |
| - | Thr 5 | deletion |
| - | Gly 6 | deletion |
| - | Ala 7 | deletion |
| - | Arg 8 | deletion |
| - | Ile 9 | deletion |
| - | Leu 10 | deletion |
| - | Ala 11 | deletion |
| - | Leu 12 | deletion |
| - | Ser 13 | deletion |
| - | Ala 14 | deletion |
| - | Leu 15 | deletion |
| - | Thr 16 | deletion |
| - | Thr 17 | deletion |
| - | Met 18 | deletion |
| - | Met 19 | deletion |
| - | Phe 20 | deletion |
| - | Ser 21 | deletion |
| - | Ala 22 | deletion |
| - | Ser 23 | deletion |
| - | Ala 24 | deletion |
| - | Leu 25 | deletion |
| - | Ala 26 | deletion |
| - | Lys 27 | deletion |
| - | Ile 28 | deletion |
| His 4 | - | expression tag |
| His 5 | - | expression tag |
| His 6 | - | expression tag |
| His 7 | - | expression tag |
| His 8 | - | expression tag |
| His 9 | - | expression tag |
| Ala 89 | Asp 108 | engineered mutation |
| Ala 90 | Lys 109 | engineered mutation |
| Ala 179 | Glu 198 | engineered mutation |
| Ala 180 | Asn 199 | engineered mutation |
| Ala 246 | Lys 265 | engineered mutation |
| Ala 366 | Glu 385 | engineered mutation |
| Ala 369 | Lys 388 | engineered mutation |
| Ala 370 | Asp 389 | engineered mutation |
| Asn 374 | Arg 393 | engineered mutation |
| Ala 375 | Ile 394 | engineered mutation |
| Ala 376 | Thr 395 | engineered mutation |
| Ala 377 | Lys 396 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 137443.2 | |
| Branched | Number of molecules | 2 |
| Total formula weight | 684.6 | |
| All* | Total formula weight | 138127.8 |
