8SAV

CryoEM structure of VRC01-CH848.0526.25

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, E, I	CH848.0526.25 gp120	polymer	487	54806.1	3	UniProt (A0A1W6IHA4) Pfam (PF00516) In PDB	HIV-1 06TG.HT008
2	B, F, J	CH848.0526.25 gp41	polymer	153	17146.5	3	Pfam (PF00517) UniProt (by SIFTS) (A0A6H1V945) In PDB	HIV-1 06TG.HT008
3	C, G, K	VRC01 variable heavy chain	polymer	121	13801.7	3		Homo sapiens (human)
4	D, H, L	VRC01 variable light chain	polymer	101	11100.2	3		Homo sapiens (human)
5	a, M, N, Q, R...	beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		586.5	9	In PDB GlyTouCan (G15407YE)
6	O, T, Y	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	3	In PDB GlyTouCan (G42666HT)
7	Z, P, U	alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		910.8	3	In PDB GlyTouCan (G22768VO)
8	A, E, I	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	6	Chemie (NAG)

Sequence modifications

A, E, I: 33 - 509 (UniProt: A0A1W6IHA4)

PDB	External Database	Details
Ala 31	-	expression tag
Glu 32	-	expression tag
Cys 201	Val 209	conflict
Cys 433	Ala 435	conflict
Lys 490	Glu 493	conflict
Glu 492	Gln 495	conflict
Val 496	Ile 499	conflict
Arg 500	Gly 503	conflict
Cys 501	Ala 504	conflict
Gly 507	Glu 510	conflict
Arg 508	Glu 512	conflict
Arg 508	Lys 513	conflict
Arg 510	-	expression tag
Arg 511	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	12
	Total formula weight	290563.6
Branched	Number of molecules	15
	Total formula weight	9284.5
Non-Polymers*	Number of molecules	6
	Total formula weight	1327.2
All*	Total formula weight	301175.4

*Water molecules are not included.