8SAQ

CryoEM structure of DH270.6-CH848.0526.25

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, E, K	CH848.0526.25 gp120	polymer	487	54806.1	3	UniProt (A0A1W6IHA4) Pfam (PF00516) In PDB	HIV-1 06TG.HT008
2	B, F, L	CH848.0526.25 gp41	polymer	153	17146.5	3	Pfam (PF00517) UniProt (by SIFTS) (A0A6H1V945) In PDB	HIV-1 06TG.HT008
3	C, H, M	DH270.6 variable heavy chain	polymer	127	14256.8	3		Homo sapiens (human)
4	D, I, N	DH270.6 variable light chain	polymer	110	11510.8	3		Homo sapiens (human)
5	G, Q, U	beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		586.5	3	In PDB GlyTouCan (G15407YE)
6	J, R, V	alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		1235.1	3	In PDB GlyTouCan (G46836GH)
7	A, E, K	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	6	Chemie (NAG)

Sequence modifications

A, E, K: 33 - 511 (UniProt: A0A1W6IHA4)

PDB	External Database	Details
Ala 31	-	expression tag
Glu 32	-	expression tag
Cys 201	Val 209	conflict
Cys 433	Ala 435	conflict
Lys 490	Glu 493	conflict
Glu 492	Gln 495	conflict
Val 496	Ile 499	conflict
Arg 500	Gly 503	conflict
Cys 501	Ala 504	conflict
Gly 507	Glu 510	conflict
Arg 509	Glu 512	conflict
Arg 510	Lys 513	conflict
Arg 512	-	expression tag
Arg 513	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	12
	Total formula weight	293160.6
Branched	Number of molecules	6
	Total formula weight	5464.9
Non-Polymers*	Number of molecules	6
	Total formula weight	1327.2
All*	Total formula weight	299952.8

*Water molecules are not included.