8SAN

CryoEM structure of VRC01-CH848.0836.10

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, E, I	CH848.0836.10 gp120	polymer	464	51860.9	3	UniProt (A0A1W6IM54) Pfam (PF00516) In PDB	HIV-1 06TG.HT008
2	B, F, J	CH848.0836.10 gp41	polymer	153	17146.5	3	Pfam (PF00517) UniProt (by SIFTS) (A0A6H1V945) In PDB	HIV-1 06TG.HT008
3	C, G, K	VCR01 variable heavy chain	polymer	224	24367.6	3		Homo sapiens (human)
4	D, H, L	VCR01 variable light chain	polymer	210	23172.7	3		Homo sapiens (human)
5	M, P, S	beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		586.5	3	In PDB GlyTouCan (G15407YE)
6	N, Q, T	alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]alpha-D-mannopyranose-(1-6)-[alpha-D-mannopyranose-(1-3)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		1235.1	3	In PDB GlyTouCan (G55220VL)
7	O, R, U	alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]alpha-D-mannopyranose-(1-6)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		1073.0	3	In PDB GlyTouCan (G94106MV)

Sequence modifications

A, E, I: 33 - 511 (UniProt: A0A1W6IM54)

PDB	External Database	Details
Ala 31	-	expression tag
Glu 32	-	expression tag
Cys 201	Val 190	conflict
Cys 433	Ala 418	conflict
Lys 490	Glu 476	conflict
Glu 492	Gln 478	conflict
Val 496	Ile 482	conflict
Arg 500	Gly 486	conflict
Cys 501	Ala 487	conflict

Contents of the asymmetric unit

Polymers	Number of chains	12
	Total formula weight	349643.2
Branched	Number of molecules	9
	Total formula weight	8683.8
All*	Total formula weight	358327.0

*Water molecules are not included.