8SAE

Crystal Structure of Cystathionine beta lyase from Klebsiella aerogenes, PLP and Hepes bound (C2 form)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Cystathionine beta-lyase	polymer	403	44102.2	2	UniProt (A0A0H3FMF8) Pfam (PF01053) In PDB	Klebsiella aerogenes KCTC 2190
2	A, B	PHOSPHATE ION	non-polymer		95.0	4	Chemie (PO4)
3	A, B	PYRIDOXAL-5'-PHOSPHATE	non-polymer		247.1	2	Chemie (PLP)
4	A, B	4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID	non-polymer		238.3	2	Chemie (EPE)
5	A, B	(4S)-2-METHYL-2,4-PENTANEDIOL	non-polymer		118.2	5	Chemie (MPD)
6		water	water		18.0	551	Chemie (HOH)

Sequence modifications

A, B: 1 - 395 (UniProt: A0A0H3FMF8)

PDB	External Database	Details
Met -7	-	initiating methionine
Ala -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
Ile 244	Val 244	engineered mutation
Pro 360	Leu 360	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	88204.5
Non-Polymers*	Number of molecules	13
	Total formula weight	1941.6
All*	Total formula weight	90146.1

*Water molecules are not included.