8SAB

Crystal Structure of Cystathionine beta lyase from Klebsiella aerogenes, PLP adduct with Alanine (C2 form)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Cystathionine beta-lyase	polymer	403	44102.2	2	UniProt (A0A0H3FMF8) Pfam (PF01053)	Klebsiella aerogenes KCTC 2190
2	C, K (A, B)	LYSINE	non-polymer		147.2	2	Chemie (LYS)
3	D, L (A, B)	ALANYL-PYRIDOXAL-5'-PHOSPHATE	non-polymer		320.2	2	Chemie (PP3)
4	E, M (A, B)	PYRIDOXAL-5'-PHOSPHATE	non-polymer		247.1	2	Chemie (PLP)
5	F, G, H, N, O... (A, B)	(4S)-2-METHYL-2,4-PENTANEDIOL	non-polymer		118.2	6	Chemie (MPD)
6	I (A)	2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL	non-polymer		122.1	1	Chemie (TRS)
7	J (A)	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
8	Q (B)	PHOSPHATE ION	non-polymer		95.0	1	Chemie (PO4)
9	R, S (A, B)	water	water		18.0	612	Chemie (HOH)

Sequence modifications

A, B: 1 - 395 (UniProt: A0A0H3FMF8)

PDB	External Database	Details
Met -7	-	initiating methionine
Ala -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
Ile 244	Val 244	engineered mutation
Pro 360	Leu 360	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	88204.5
Non-Polymers*	Number of molecules	15
	Total formula weight	2447.4
All*	Total formula weight	90651.9

*Water molecules are not included.