8SA9

Crystal Structure of Cystathionine beta lyase from Klebsiella aerogenes, PLP-Oxamate Adduct (C2 form)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Cystathionine beta-lyase	polymer	403	44102.2	2	UniProt (A0A0H3FMF8) Pfam (PF01053)	Klebsiella aerogenes KCTC 2190
2	C, F (A, B)	[({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)amino](oxo)acetic acid	non-polymer		320.2	2	Chemie (ZTS)
3	D, G (A, B)	PYRIDOXAL-5'-PHOSPHATE	non-polymer		247.1	2	Chemie (PLP)
4	E (B)	CITRATE ANION	non-polymer		189.1	1	Chemie (FLC)
5	H, I (A, B)	water	water		18.0	988	Chemie (HOH)

Sequence modifications

A, B: 1 - 395 (UniProt: A0A0H3FMF8)

PDB	External Database	Details
Met -7	-	initiating methionine
Ala -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
Ile 244	Val 244	engineered mutation
Pro 360	Leu 360	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	88204.5
Non-Polymers*	Number of molecules	5
	Total formula weight	1323.8
All*	Total formula weight	89528.3

*Water molecules are not included.