8S9V
CRISPR-Cas type III-D effector complex bound to a self-target RNA in the pre-cleavage state
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A | Cas7-Cas5-Cas11 | polymer | 791 | 87558.9 | 1 | UniProt (Q6ZED2) Pfam (PF03787) In PDB | Synechocystis sp. PCC 6803 | |
2 | B | TIGR03984 family CRISPR-associated protein | polymer | 193 | 21899.8 | 1 | UniProt (Q6ZED4) In PDB | Synechocystis sp. PCC 6803 | |
3 | C | Cas10 | polymer | 575 | 64335.3 | 1 | UniProt (Q6ZED1) Pfam (PF22335) In PDB | Synechocystis sp. PCC 6803 | |
4 | D | Cas7-2x | polymer | 522 | 56820.8 | 1 | UniProt (Q6ZED3) Pfam (PF03787) In PDB | Synechocystis sp. PCC 6803 | |
5 | E | TIGR03986 family CRISPR-associated RAMP protein | polymer | 795 | 90335.0 | 1 | UniProt (Q6ZED5) Pfam (PF03787) In PDB | Synechocystis sp. PCC 6803 | |
6 | F | CRISPR RNA | polymer | 37 | 11927.2 | 1 | Synechocystis sp. PCC 6803 | ||
7 | G | Self-target RNA | polymer | 60 | 19170.3 | 1 | Synechocystis sp. PCC 6803 | ||
8 | A, D, G | MAGNESIUM ION | non-polymer | 24.3 | 5 | Chemie (MG) | |||
9 | water | water | 18.0 | 19 | Chemie (HOH) |
Sequence modifications
C: 2 - 558 (UniProt: Q6ZED1)
PDB | External Database | Details |
---|---|---|
Met -16 | - | initiating methionine |
Ala -15 | - | expression tag |
His -14 | - | expression tag |
His -13 | - | expression tag |
His -12 | - | expression tag |
His -11 | - | expression tag |
His -10 | - | expression tag |
His -9 | - | expression tag |
Val -8 | - | expression tag |
Gly -7 | - | expression tag |
Thr -6 | - | expression tag |
Glu -5 | - | expression tag |
Asn -4 | - | expression tag |
Leu -3 | - | expression tag |
Tyr -2 | - | expression tag |
Phe -1 | - | expression tag |
Gln 0 | - | expression tag |
Gly 1 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 7 |
Total formula weight | 352047.4 | |
Non-Polymers* | Number of molecules | 5 |
Total formula weight | 121.5 | |
All* | Total formula weight | 352168.9 |