8S9V

CRISPR-Cas type III-D effector complex bound to a self-target RNA in the pre-cleavage state

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Cas7-Cas5-Cas11	polymer	791	87558.9	1	UniProt (Q6ZED2) Pfam (PF03787)	Synechocystis sp. PCC 6803
2	B (B)	TIGR03984 family CRISPR-associated protein	polymer	193	21899.8	1	UniProt (Q6ZED4)	Synechocystis sp. PCC 6803
3	C (C)	Cas10	polymer	575	64335.3	1	UniProt (Q6ZED1) Pfam (PF22335)	Synechocystis sp. PCC 6803
4	D (D)	Cas7-2x	polymer	522	56820.8	1	UniProt (Q6ZED3) Pfam (PF03787)	Synechocystis sp. PCC 6803
5	E (E)	TIGR03986 family CRISPR-associated RAMP protein	polymer	795	90335.0	1	UniProt (Q6ZED5) Pfam (PF03787)	Synechocystis sp. PCC 6803
6	F (F)	CRISPR RNA	polymer	37	11927.2	1		Synechocystis sp. PCC 6803
7	G (G)	Self-target RNA	polymer	60	19170.3	1		Synechocystis sp. PCC 6803
8	H, I, J, K, L (A, D, G)	MAGNESIUM ION	non-polymer		24.3	5	Chemie (MG)
9	M, N, O (A, D, G)	water	water		18.0	19	Chemie (HOH)

Sequence modifications

C: 2 - 558 (UniProt: Q6ZED1)

PDB	External Database	Details
Met -16	-	initiating methionine
Ala -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
Val -8	-	expression tag
Gly -7	-	expression tag
Thr -6	-	expression tag
Glu -5	-	expression tag
Asn -4	-	expression tag
Leu -3	-	expression tag
Tyr -2	-	expression tag
Phe -1	-	expression tag
Gln 0	-	expression tag
Gly 1	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	7
	Total formula weight	352047.4
Non-Polymers*	Number of molecules	5
	Total formula weight	121.5
All*	Total formula weight	352168.9

*Water molecules are not included.