8S8I

Structure of a yeast 48S-AUC preinitiation complex in closed conformation (model py48S-AUC-eIF1)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	2	18S ribosomal RNA	polymer	1798	579454.9	1		Kluyveromyces lactis NRRL Y-1140
2	A	Small ribosomal subunit protein uS2	polymer	254	28264.5	1	UniProt (Q6CN12) In PDB	Kluyveromyces lactis NRRL Y-1140	40S ribosomal protein S0
3	B	Small ribosomal subunit protein eS1	polymer	255	28971.6	1	UniProt (Q6CWD0) In PDB	Kluyveromyces lactis NRRL Y-1140	40S ribosomal protein S1
4	C	Small ribosomal subunit protein uS5	polymer	259	27650.0	1	UniProt (Q6CKL3) In PDB	Kluyveromyces lactis NRRL Y-1140	40S ribosomal protein S2
5	E	40S ribosomal protein S4	polymer	261	29617.5	1	UniProt (Q6CWJ2) In PDB	Kluyveromyces lactis NRRL Y-1140
6	G	Small ribosomal subunit protein eS6	polymer	236	26970.4	1	UniProt (Q6CM04) In PDB	Kluyveromyces lactis NRRL Y-1140	40S ribosomal protein S6
7	H	40S ribosomal protein S7	polymer	190	21735.3	1	UniProt (Q6CTD6) In PDB	Kluyveromyces lactis NRRL Y-1140
8	I	40S ribosomal protein S8	polymer	201	22642.7	1	UniProt (Q6CMG3) In PDB	Kluyveromyces lactis NRRL Y-1140
9	J	KLLA0E23673p	polymer	188	21587.0	1	UniProt (Q6CM18) In PDB	Kluyveromyces lactis NRRL Y-1140
10	L	KLLA0A10483p	polymer	156	17843.9	1	UniProt (Q6CX80) In PDB	Kluyveromyces lactis NRRL Y-1140
11	N	KLLA0F18040p	polymer	151	16989.9	1	UniProt (Q6CJK0) In PDB	Kluyveromyces lactis NRRL Y-1140
12	O	Small ribosomal subunit protein uS11	polymer	137	14530.7	1	UniProt (P27069) In PDB	Kluyveromyces lactis NRRL Y-1140	40S ribosomal protein S14,RP59
13	V	40S ribosomal protein S21	polymer	87	9797.9	1	UniProt (Q6CXT6) In PDB	Kluyveromyces lactis NRRL Y-1140
14	W	Small ribosomal subunit protein uS8	polymer	130	14645.0	1	UniProt (Q6CW21) In PDB	Kluyveromyces lactis NRRL Y-1140	40S ribosomal protein S22
15	X	KLLA0B11231p	polymer	145	16047.9	1	UniProt (F2Z602) In PDB	Kluyveromyces lactis NRRL Y-1140
16	Y	40S ribosomal protein S24	polymer	135	15194.5	1	UniProt (Q6CU44) In PDB	Kluyveromyces lactis NRRL Y-1140
17	a	40S ribosomal protein S26	polymer	119	13540.0	1	UniProt (Q6CS01) In PDB	Kluyveromyces lactis NRRL Y-1140
18	b	40S ribosomal protein S27	polymer	82	8884.4	1	UniProt (Q6CNL2) In PDB	Kluyveromyces lactis NRRL Y-1140
19	e	40S ribosomal protein S30	polymer	63	7141.4	1	UniProt (Q6CUH5) In PDB	Kluyveromyces lactis NRRL Y-1140
20	h	40S ribosomal protein L41-A	polymer	25	3354.2	1		Kluyveromyces lactis NRRL Y-1140
21	D	40S ribosomal protein S3	polymer	237	26300.5	1	UniProt (Q6CRK7) In PDB	Kluyveromyces lactis NRRL Y-1140
22	F	KLLA0D10659p	polymer	227	25386.0	1	UniProt (Q6CRA3) In PDB	Kluyveromyces lactis NRRL Y-1140
23	K	KLLA0B08173p	polymer	106	12584.4	1	UniProt (Q6CVZ5) In PDB	Kluyveromyces lactis NRRL Y-1140
24	M	40S ribosomal protein S12	polymer	134	14466.4	1	UniProt (Q6CLU4) In PDB	Kluyveromyces lactis NRRL Y-1140
25	P	KLLA0F07843p	polymer	142	15986.8	1	UniProt (Q6CKV4) In PDB	Kluyveromyces lactis NRRL Y-1140
26	Q	Small ribosomal subunit protein uS9	polymer	143	15874.5	1	UniProt (Q875N2) In PDB	Kluyveromyces lactis NRRL Y-1140	40S ribosomal protein S16
27	R	KLLA0B01474p	polymer	136	15722.2	1	UniProt (Q6CWU3) In PDB	Kluyveromyces lactis NRRL Y-1140
28	S	KLLA0B01562p	polymer	146	17084.6	1	UniProt (Q6CWT9) In PDB	Kluyveromyces lactis NRRL Y-1140
29	T	KLLA0A07194p	polymer	144	15879.0	1	UniProt (Q6CXM0) In PDB	Kluyveromyces lactis NRRL Y-1140
30	U	Small ribosomal subunit protein uS10	polymer	117	13337.6	1	UniProt (Q6CIM1) In PDB	Kluyveromyces lactis NRRL Y-1140	40S ribosomal protein S20
31	Z	40S ribosomal protein S25	polymer	108	12002.1	1	UniProt (Q6CW78) In PDB	Kluyveromyces lactis NRRL Y-1140
32	c	Small ribosomal subunit protein eS28	polymer	67	7549.8	1	UniProt (P33285) In PDB	Kluyveromyces lactis NRRL Y-1140	40S ribosomal protein S28,S33
33	d	Small ribosomal subunit protein uS14	polymer	56	6662.6	1	UniProt (Q6CPG3) In PDB	Kluyveromyces lactis NRRL Y-1140	40S ribosomal protein S29
34	f	Small ribosomal subunit protein eS31	polymer	150	17111.0	1	UniProt (P69061) In PDB	Kluyveromyces lactis NRRL Y-1140	40S ribosomal protein S27a
35	g	KLLA0E12277p	polymer	326	35830.9	1	UniProt (Q6CNI7) In PDB	Kluyveromyces lactis NRRL Y-1140
36	i	Eukaryotic translation initiation factor 1A	polymer	153	17462.2	1	UniProt (P38912) In PDB	Saccharomyces cerevisiae S288C	eIF-1A,Eukaryotic translation initiation factor 4C,eIF-4C
37	3	mRNA (5'-R(P*AP*AP*U)-3')	polymer	49	15304.9	1		Kluyveromyces lactis
38	1	Met-tRNAi	polymer	75	24714.0	1		Saccharomyces cerevisiae S288C
39	j	Eukaryotic translation initiation factor 2 subunit alpha	polymer	304	34763.7	1	UniProt (P20459) In PDB	Saccharomyces cerevisiae S288C	eIF-2-alpha,eIF-2A,eIF-2alpha,eIF2-alpha
40	k	Eukaryotic translation initiation factor 2 subunit gamma	polymer	527	57942.7	1	UniProt (P32481) In PDB	Saccharomyces cerevisiae S288C	eIF2-gamma
41	l	Eukaryotic translation initiation factor 2 subunit beta	polymer	285	31631.3	1	UniProt (P09064) In PDB	Saccharomyces cerevisiae S288C	eIF2-beta
42	m	Eukaryotic translation initiation factor eIF-1	polymer	108	12330.1	1	UniProt (P32911) In PDB	Saccharomyces cerevisiae S288C	Protein translation factor SUI1
43	2, S, i, k	MAGNESIUM ION	non-polymer		24.3	117	Chemie (MG)
44	a, b, f, l	ZINC ION	non-polymer		65.4	4	Chemie (ZN)
45	k	PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER	non-polymer		521.2	1	Chemie (GCP)
46	k	METHIONINE	non-polymer		149.2	1	Chemie (MET)
47		water	water		18.0	3	Chemie (HOH)

Contents of the asymmetric unit

Polymers	Number of chains	42
	Total formula weight	1370791.2
Non-Polymers*	Number of molecules	123
	Total formula weight	3775.7
All*	Total formula weight	1374567.0

*Water molecules are not included.