8S5A

The crystal structure of FAN1 Nuclease bound to 5' phosphorylated p(dG)/3'(dT-dT-dT-dT) double flap DNA

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Fanconi-associated nuclease 1	polymer	654	74162.3	1	UniProt (Q9Y2M0) Pfam (PF21315) Pfam (PF21169) Pfam (PF21170) Pfam (PF08774)	Homo sapiens (human)	FANCD2/FANCI-associated nuclease 1,hFAN1,Myotubularin-related protein 15
2	B (B)	DNA (5'-D(*AP*AP*CP*AP*CP*GP*CP*CP*TP*AP*GP*AP*CP*TP*CP*CP*TP*CP*A)-3')	polymer	19	5718.7	1		synthetic construct
3	C (C)	DNA (5'-D(P*GP*AP*GP*GP*CP*GP*TP*G)-3')	polymer	8	2507.7	1		synthetic construct
4	D (D)	DNA (5'-D(*TP*TP*TP*GP*AP*GP*GP*AP*GP*TP*CP*TP*T)-3')	polymer	16	4925.2	1		synthetic construct
5	E, F (A)	CALCIUM ION	non-polymer		40.1	2	Chemie (CA)
6	G (C)	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL)
7	H, I (A, B)	water	water		18.0	16	Chemie (HOH)

Sequence modifications

A: 364 - 1017 (UniProt: Q9Y2M0)

PDB	External Database	Details
Gly 355	-	expression tag
Pro 356	-	expression tag
Met 357	-	expression tag
Gly 358	-	expression tag
Ala 359	-	expression tag
His 360	-	expression tag
Met 361	-	expression tag
Thr 362	-	expression tag
Arg 363	-	expression tag
His 507	Arg 507	conflict
-	Cys 510	deletion
-	Thr 511	deletion
-	Trp 512	deletion
-	Gly 513	deletion
-	Lys 514	deletion
-	Asn 515	deletion
-	Lys 516	deletion
-	Pro 517	deletion
-	Gly 518	deletion
Ala 794	Lys 794	conflict

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	87313.9
Non-Polymers*	Number of molecules	3
	Total formula weight	115.6
All*	Total formula weight	87429.5

*Water molecules are not included.