8S35
DNA-bound Type IV-A3 CRISPR effector in complex with DinG helicase from K. pneumoniae (state I)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D, E... (A, B, C, D, E...) | CRISPR type AFERR-associated protein Csf2 | polymer | 350 | 38654.4 | 6 | UniProt (A0A333ESG5) | Klebsiella pneumoniae | Csf2 (Cas7), CRISPR-Cas type IV-A3 Cas7 (Csf2) |
| 2 | F (F) | CRISPR type AFERR-associated protein Csf3 | polymer | 235 | 25474.4 | 1 | UniProt (A0A8G1XN67) | Klebsiella pneumoniae | Csf3 (Cas5), CRISPR-Cas type IV-A3 Cas5 (Csf3) |
| 3 | G (G) | CRISPR type AFERR-associated protein Csf1 | polymer | 263 | 29762.5 | 1 | UniProt (A0A7Z7WW72) | Klebsiella pneumoniae | Csf1 (Cas8), CRISPR-Cas type IV-A3 Cas8 (Csf1) |
| 4 | H (H) | crRNA | polymer | 61 | 19672.6 | 1 | Klebsiella pneumoniae | ||
| 5 | I (I) | TS-DNA | polymer | 60 | 18196.6 | 1 | Klebsiella pneumoniae | ||
| 6 | J (J) | NTS-DNA | polymer | 60 | 18743.0 | 1 | Klebsiella pneumoniae | ||
| 7 | L (M) | DEAD/DEAH box helicase | polymer | 624 | 70114.5 | 1 | UniProt (A0A422ZM74) Pfam (PF00270) Pfam (PF13307) | Klebsiella pneumoniae | CRISPR-Cas type IV-A3 DinG (Csf4) |
| 8 | M (G) | ZINC ION | non-polymer | 65.4 | 1 | Chemie (ZN) |
Sequence modifications
A, B, C, D, E, L: 1 - 343 (UniProt: A0A333ESG5)
F: 1 - 235 (UniProt: A0A8G1XN67)
M: 1 - 624 (UniProt: A0A422ZM74)
| PDB | External Database | Details |
|---|---|---|
| Gly 344 | - | expression tag |
| His 345 | - | expression tag |
| His 346 | - | expression tag |
| His 347 | - | expression tag |
| His 348 | - | expression tag |
| His 349 | - | expression tag |
| His 350 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Met 84 | Val 84 | conflict |
| Thr 103 | Ile 103 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Glu 292 | Lys 292 | conflict |
| Asn 412 | Asp 412 | conflict |
| Gly 421 | Asp 421 | conflict |
| Gln 435 | His 435 | conflict |
| Phe 618 | Cys 618 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 12 |
| Total formula weight | 413890.0 | |
| Non-Polymers* | Number of molecules | 1 |
| Total formula weight | 65.4 | |
| All* | Total formula weight | 413955.4 |
