8S2T
Cryo-EM structure of cell-free synthesized Human beta-1 adrenergic receptor cotranslationally inserted into a lipidic nanodiscs
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Isoform Gnas-2 of Guanine nucleotide-binding protein G(s) subunit alpha isoforms short | polymer | 380 | 44326.2 | 1 | UniProt (P63092) Pfam (PF00503) | Homo sapiens (human) | Adenylate cyclase-stimulating G alpha protein |
| 2 | B (B) | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | polymer | 345 | 37728.2 | 1 | UniProt (P62873) Pfam (PF00400) | Homo sapiens (human) | Transducin beta chain 1 |
| 3 | C (C) | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | polymer | 68 | 7563.8 | 1 | UniProt (P59768) Pfam (PF00631) | Homo sapiens (human) | G gamma-I |
| 4 | D (N) | Nanobody-35 | polymer | 134 | 14714.3 | 1 | Lama glama | ||
| 5 | E (R) | Beta-1 adrenergic receptor | polymer | 477 | 51130.3 | 1 | UniProt (P08588) Pfam (PF00001) | Homo sapiens (human) | Beta-1 adrenoreceptor,Beta-1 adrenoceptor |
| 6 | F (R) | ISOPRENALINE | non-polymer | 211.3 | 1 | Chemie (5FW) |
Sequence modifications
B: 2 - 340 (UniProt: P62873)
R: 1 - 477 (UniProt: P08588)
| PDB | External Database | Details |
|---|---|---|
| Gly -4 | - | expression tag |
| Pro -3 | - | expression tag |
| Gly -2 | - | expression tag |
| Ser -1 | - | expression tag |
| Ser 0 | - | expression tag |
| Gly 1 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Ser 85 | Lys 85 | conflict |
| Val 107 | Met 107 | conflict |
| Val 146 | Ile 146 | conflict |
| Trp 147 | Glu 147 | conflict |
| Leu 316 | Ala 316 | conflict |
| Ala 361 | Phe 361 | conflict |
| Met 372 | Phe 372 | conflict |
| Ala 392 | Cys 392 | conflict |
| Ala 393 | Cys 393 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 5 |
| Total formula weight | 155462.7 | |
| Non-Polymers* | Number of molecules | 1 |
| Total formula weight | 211.3 | |
| All* | Total formula weight | 155674.0 |
