8RXJ
Desulfovibrio desulfuricans [FeFe] hydrogenase C178A mutant in Hinact-like state derivatized with krypton
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Periplasmic [Fe] hydrogenase large subunit | polymer | 405 | 44365.0 | 1 | UniProt (P07598) Pfam (PF00037) Pfam (PF02906) | Desulfovibrio desulfuricans | Fe hydrogenlyase |
| 2 | B (B) | Periplasmic [Fe] hydrogenase small subunit | polymer | 88 | 10082.4 | 1 | UniProt (P07603) Pfam (PF02256) | Desulfovibrio desulfuricans | Fe hydrogenlyase small chain |
| 3 | C, D, E, F, G... (A) | KRYPTON | non-polymer | 83.8 | 10 | Chemie (KR) | |||
| 4 | M (A) | dicarbonyl[bis(cyanide-kappaC)]-mu-(iminodimethanethiolatato-1kappaS:2kappaS)-mu-(oxomethylidene)diiron(2+) | non-polymer | 355.0 | 1 | Chemie (402) | |||
| 5 | N, O, P (A) | IRON/SULFUR CLUSTER | non-polymer | 351.6 | 3 | Chemie (SF4) | |||
| 6 | Q (A) | CYANIDE ION | non-polymer | 26.0 | 1 | Chemie (CYN) | |||
| 7 | R (A) | 2-[2-(2-METHOXY-ETHOXY)-ETHOXY]-ETHOXYL | non-polymer | 164.2 | 1 | Chemie (TOE) | |||
| 8 | S, T (A, B) | water | water | 18.0 | 220 | Chemie (HOH) |
Sequence modifications
A: 1 - 397 (UniProt: P07598)
| PDB | External Database | Details |
|---|---|---|
| Ala 178 | Cys 178 | engineered mutation |
| Trp 398 | - | expression tag |
| Ser 399 | - | expression tag |
| His 400 | - | expression tag |
| Pro 401 | - | expression tag |
| Gln 402 | - | expression tag |
| Phe 403 | - | expression tag |
| Glu 404 | - | expression tag |
| Lys 405 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 54447.5 | |
| Non-Polymers* | Number of molecules | 16 |
| Total formula weight | 2438.1 | |
| All* | Total formula weight | 56885.5 |
